Tamura Y, Suzuki S, Sawada T
National Veterinary Assay Laboratory, Tokyo, Japan.
Microb Pathog. 1992 Mar;12(3):237-44. doi: 10.1016/0882-4010(92)90058-v.
The role of elastase and alkaline protease in the pathogenesis of fatal infections caused by Pseudomonas aeruginosa was determined in mice treated with calcium chloride. Mortality increased significantly when solutions containing elastase were injected together with non-lethal inocula of strain PA 103, which does not produce proteolytic enzyme. In contrast, solutions containing alkaline protease did not increase mortality. In mice injected intramuscularly with strain PA 103 and calcium chloride, the organisms grew rapidly in the injected muscle but not in the liver. However, when elastase was injected together with strain PA 103 and calcium chloride, viable bacteria were also found in the liver. Moreover, the survival rate of mice challenged with elastase-producing strain 5 and calcium chloride was enhanced, and colonization of the liver prevented, by immunization with elastase toxoid. These results suggest that elastase contributes to the invasiveness of the organism.
在用氯化钙处理的小鼠中，测定了弹性蛋白酶和碱性蛋白酶在铜绿假单胞菌所致致命感染发病机制中的作用。当将含有弹性蛋白酶的溶液与不产生蛋白水解酶的PA 103菌株的非致死性接种物一起注射时，死亡率显著增加。相比之下，含有碱性蛋白酶的溶液并未增加死亡率。在肌肉注射PA 103菌株和氯化钙的小鼠中，细菌在注射的肌肉中迅速生长，但在肝脏中不生长。然而，当弹性蛋白酶与PA 103菌株和氯化钙一起注射时，在肝脏中也发现了活细菌。此外，用弹性蛋白酶类毒素免疫可提高用产弹性蛋白酶的5菌株和氯化钙攻击的小鼠的存活率，并防止肝脏定植。这些结果表明弹性蛋白酶有助于该生物体的侵袭性。
