Porter K R, Mascola J R, Hupudio H, Ewing D, VanCott T C, Anthony R L, Corwin A L, Widodo S, Ertono S, McCutchan F E, Burke D S, Hayes C G, Wignall F S, Graham R R
Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland, USA.
J Acquir Immune Defic Syndr Hum Retrovirol. 1997 Jan 1;14(1):1-6. doi: 10.1097/00042560-199701010-00001.
To examine the genetic and antigenic characteristics of HIV-1 in Indonesia, samples from 19 HIV-positive volunteers were studied. By a combination of PCR typing and DNA sequence analysis, 12 of the 19 volunteers were determined to be infected with HIV-1 clade B and seven with clade E. Six of the seven Indonesian clade E isolates were from volunteers associated with the Indonesian Military during a peacekeeping mission in Cambodia. Infectivity reduction neutralization assays showed that the Indonesian E viruses were effectively neutralized by Thailand clade E HIV-1 antisera but not by U.S. clade B antisera. The Indonesian clade B virus tested was neutralized by U.S. clade B antisera and not by the Thailand E antisera. Using a previously described serologic typing ELISA based on clade B and E V3 peptides, genetic clade was accurately determined in eight of eight sera tested. This is the first report of the genetic and antigenic analysis of HIV-1 isolates from Indonesia. The data indicate that at least two genetic and antigenic HIV-1 clades (clade E and B) circulate in Indonesia.
为研究印度尼西亚HIV-1的基因和抗原特性，对19名HIV阳性志愿者的样本进行了研究。通过聚合酶链反应（PCR）分型和DNA序列分析相结合的方法，确定19名志愿者中有12人感染了HIV-1 B亚型，7人感染了E亚型。7株印度尼西亚E亚型分离株中的6株来自在柬埔寨执行维和任务期间与印尼军方有关联的志愿者。感染性降低中和试验表明，印度尼西亚E亚型病毒能被泰国E亚型HIV-1抗血清有效中和，但不能被美国B亚型抗血清中和。所检测的印度尼西亚B亚型病毒能被美国B亚型抗血清中和，而不能被泰国E亚型抗血清中和。使用先前描述的基于B亚型和E亚型V3肽的血清学分型酶联免疫吸附测定（ELISA），在检测的8份血清中有8份准确确定了基因亚型。这是关于印度尼西亚HIV-1分离株基因和抗原分析的首次报告。数据表明，至少两种基因和抗原不同的HIV-1亚型（E亚型和B亚型）在印度尼西亚传播。
