通过抗体结合和中和作用对泰国感染HIV-1 B亚型和E亚型的受试者血清进行特征分析。

Characterization of sera from subjects infected with HIV-1 subtypes B and E in Thailand by antibody binding and neutralization.

作者信息

Louisirirotchanakul S, Beddows S, Cheingsong-Popov R, Shaffer N, Mastro T D, Auewarakul P, Likanonsakul S, Wasi C, Weber J

机构信息

Department of Genito-Urinary Medicine and Communicable Diseases, Imperial College School of Medicine at St Mary's, London, UK.

出版信息

J Acquir Immune Defic Syndr Hum Retrovirol. 1998 Dec 1;19(4):315-20. doi: 10.1097/00042560-199812010-00001.

DOI:10.1097/00042560-199812010-00001

PMID:9833739

Abstract

The range and specificity of the humoral immune response to HIV-1 subtypes B and E was investigated in Thai samples. Sera from HIV-1-positive subjects, consisting of subtypes B (n = 24) and E (n = 138), were characterized in relation to the neutralization of primary isolates and T-cell line-adapted (TCLA) strains and binding to monomeric gp120, the CD4/gp120 binding site (BS), and V3 peptides. A subtype-specific pattern of antibody binding was observed with the exception of the CD4/gp 120MN BS. Neutralization of TCLA strains (n = 4) was strongly type-specific (p = .002); however, neutralization of primary isolates (n = 8) was weak and group specific. Thus, the subtype specificity of B and E sera in the neutralization of TCLA strains, but not primary isolates, supports the dominance of the V3 region in TCLA virus neutralization but does not support the distinction of subtypes B and E as discrete neutralization serotypes in Thailand.

摘要

在泰国样本中研究了针对HIV-1 B亚型和E亚型的体液免疫反应的范围和特异性。对来自HIV-1阳性受试者的血清进行了特征分析，这些血清包括B亚型（n = 24）和E亚型（n = 138），分析内容涉及对原始分离株和T细胞系适应株（TCLA）的中和作用以及与单体gp120、CD4/gp120结合位点（BS）和V3肽的结合情况。除了CD4/gp120MN BS外，观察到了抗体结合的亚型特异性模式。对TCLA毒株（n = 4）的中和作用具有很强的型特异性（p = .002）；然而，对原始分离株（n = 8）的中和作用较弱且具有组特异性。因此，B亚型和E亚型血清在中和TCLA毒株而非原始分离株方面的亚型特异性，支持了V3区域在TCLA病毒中和中的主导地位，但不支持将B亚型和E亚型区分为泰国不同的中和血清型。