Yuzbasiyan-Gurkan V, Blanton S H, Cao Y, Ferguson P, Li J, Venta P J, Brewer G J
Department of Small Animal Clinical Sciences, Michigan State University, East Lansing 48824, USA.
Am J Vet Res. 1997 Jan;58(1):23-7.
To identify a DNA marker for the copper toxicosis (CT) locus in Bedlington Terriers (BT).
77 BT, of which 25 were affected. Diagnosis of affected or unaffected with CT was made in all cases by quantitative copper determinations on liver biopsy samples by use of established criteria.
BT pedigrees segregating for CT were identified. Linkage studies were carried out using polymorphic microsatellite markers developed for the canine genome in these pedigrees. DNA was isolated from blood samples of pedigree members. Polymerase chain reaction was used to amplify and type alleles at 213 microsatellite loci in each dog, and findings were subjected to linkage analysis.
One microsatellite marker was identified to be closely linked to CT with logarithm of odds score of 5.96 at a recombination fraction of zero.
Using the linked marker, it has become possible to distinguish affected, homozygous normal, and carrier dogs in some BT pedigrees.
In informative pedigrees where the marker is variable in the parents, it is possible to identify which dogs will require anticopper therapy and provide breeders with sound scientific advice about breeding strategies.
鉴定贝林登梗犬（BT）铜中毒（CT）基因座的DNA标记。
77只BT，其中25只患病。所有病例均通过对肝活检样本进行定量铜测定，并采用既定标准诊断是否患有CT。
确定了CT基因分离的BT系谱。在这些系谱中，使用为犬基因组开发的多态微卫星标记进行连锁研究。从系谱成员的血液样本中分离DNA。利用聚合酶链反应对每只犬的213个微卫星位点的等位基因进行扩增和分型，并对结果进行连锁分析。
鉴定出一个微卫星标记与CT紧密连锁，在重组率为零时，优势对数得分为5.96。
利用该连锁标记，在一些BT系谱中已能够区分患病犬、纯合正常犬和携带犬。
在标记在亲代中可变的信息丰富的系谱中，有可能确定哪些犬需要进行抗铜治疗，并为育种者提供有关育种策略的可靠科学建议。
