Onodera H, Nakamura S, Sugai T
Department of Dermatology, Iwate Medical University, Morioka, Japan.
Am J Dermatopathol. 1996 Dec;18(6):580-8. doi: 10.1097/00000372-199612000-00005.
We investigated correlations between cell proliferation, p53 overexpression, and degree of malignancy in cutaneous epithelial neoplasms. One hundred and fourteen cases of epithelial neoplasms, including seborrheic keratosis (SEB), basal cell carcinomas (BCCs), solar keratosis (SK), Bowen's disease (BD), and squamous cell carcinomas (SCCs) were examined using argyrophilic nucleolar organizer region (AgNOR) staining. In addition, immunohistochemical analysis using the Ki-67 (MIB-1) and anti-p53 (DO-7) monoclonal antibodies was performed. The ratio of tumorous to normal cells according to AgNOR staining was defined as the AgNOR rate, and the ratio of tumorous to normal cells according to Ki-67 recognition was defined as the Ki-67 rate. SCC lesions showed the highest AgNOR rate among the investigated epithelial neoplasms, followed in order by BD, BCC, SK, and SEB lesions. The Ki-67 rate was highest in BD lesions, followed in order by SK, SCC, BCC, and SEB lesions. Expression of p53 protein was highest in SK lesions. SCC is generally considered to be the most malignant neoplasm, followed in order by BCC, BD, and SK. Thus, our results suggest that the Ki-67 rate and overexpression of p53 protein do not always reflect the degree of malignancy in neoplasms.
我们研究了皮肤上皮性肿瘤中细胞增殖、p53过表达与恶性程度之间的相关性。使用嗜银核仁组成区（AgNOR）染色法检查了114例上皮性肿瘤，包括脂溢性角化病（SEB）、基底细胞癌（BCC）、日光性角化病（SK）、鲍温病（BD）和鳞状细胞癌（SCC）。此外，还使用Ki-67（MIB-1）和抗p53（DO-7）单克隆抗体进行了免疫组化分析。根据AgNOR染色得出的肿瘤细胞与正常细胞的比例定义为AgNOR率，根据Ki-67识别得出的肿瘤细胞与正常细胞的比例定义为Ki-67率。在研究的上皮性肿瘤中，SCC病变的AgNOR率最高，其次依次为BD、BCC、SK和SEB病变。BD病变的Ki-67率最高，其次依次为SK、SCC、BCC和SEB病变。p53蛋白的表达在SK病变中最高。SCC通常被认为是最恶性的肿瘤，其次依次为BCC、BD和SK。因此，我们的结果表明，Ki-67率和p53蛋白的过表达并不总是反映肿瘤的恶性程度。
