酿酒酵母中依赖烟酰胺腺嘌呤二核苷酸的5,10-亚甲基四氢叶酸脱氢酶的结晶
Crystallization of the NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase from Saccharomyces cerevisiae.
作者信息
Monzingo A F, West M G, Schelp E, Appling D R, Robertus J D
机构信息
Department of Chemistry and Biochemistry, University of Texas at Austin 78712, USA.
出版信息
Proteins. 1996 Dec;26(4):481-2. doi: 10.1002/(SICI)1097-0134(199612)26:4<481::AID-PROT10>3.0.CO;2-J.
Saccharomyces cerevisiae possesses three isozymes of 5,10-methylenetetrahydrofolate dehydrogenase (MTD). The NAD-dependent enzyme is the first monofunctional form found in eukaryotes. Here we report its crystallization in a form suitable for high-resolution structure. The space group is P4(2)2(1)2 with cell constants a = b = 75.9, c = 160.0 A, and there is one 36 kDa molecule in the asymmetric unit. Crystals diffract to 2.9 A resolution.
酿酒酵母拥有三种5,10-亚甲基四氢叶酸脱氢酶（MTD）同工酶。这种依赖NAD的酶是在真核生物中发现的第一种单功能形式。在此，我们报告了其适合高分辨率结构分析的晶体形式。空间群为P4(2)2(1)2，晶胞参数a = b = 75.9，c = 160.0 Å，不对称单元中有一个36 kDa的分子。晶体衍射分辨率达到2.9 Å。
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