Bhuiya Mohammad W, Sakuraba Haruhiko, Yoneda Kazunari, Ohshima Toshihisa, Imagawa Takahito, Katunuma Nobuhiko, Tsuge Hideaki
Department of Biological Science and Technology, Faculty of Engineering,The University of Tokushima, 2-1 Minamijosanjima-cho, Tokushima 770-8506, Japan.
Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):715-7. doi: 10.1107/S0907444904001854. Epub 2004 Mar 23.
NAD-dependent glutamate dehydrogenase from the hyperthermophilic archaeon Pyrobaculum islandicum was crystallized in the apo- and holoenzyme forms. Crystals were obtained using 2-propanol and polyethylene glycol MME 550 as precipitants for the apoenzyme and holoenzyme, respectively. The apoenzyme crystals belong to the trigonal space group P3(1)21 or its enantiomorph P3(2)21. The asymmetric unit contains three subunits; the values of the Matthews coefficient (VM) and the solvent content are 2.9 A3 Da-1 and 57%, respectively. A native data set was collected to a highest resolution limit of 4.0 A on an in-house X-ray source using a rotating-anode generator (overall Rsym of 12.3% and completeness of 97%). The holoenzyme crystals belong to the orthorhombic space group P2(1)2(1)2(1); the asymmetric unit contains one hexamer, giving a VM of 2.79 A3 Da-1 and a solvent content of 55%. Native and derivative data sets were collected. The crystals diffract to a maximum resolution of 2.8 A on the KEK-NW12 beamline at the Photon Factory and gave a data set with an overall Rsym of 7.9% and a completeness of 91%. Attempts are being made to solve the structure by the SIRAS method.
嗜热古菌冰岛硫化叶菌的NAD依赖型谷氨酸脱氢酶以脱辅基酶和全酶形式结晶。分别使用2-丙醇和聚乙二醇MME 550作为脱辅基酶和全酶的沉淀剂获得晶体。脱辅基酶晶体属于三方晶系空间群P3(1)21或其对映体P3(2)21。不对称单元包含三个亚基；马修斯系数（VM）值和溶剂含量分别为2.9 ų Da⁻¹和57%。使用旋转阳极发生器在内部X射线源上收集了一个天然数据集，最高分辨率极限为4.0 Å（总体Rsym为12.3%，完整性为97%）。全酶晶体属于正交晶系空间群P2(1)2(1)2(1)；不对称单元包含一个六聚体，VM为2.79 ų Da⁻¹，溶剂含量为55%。收集了天然和衍生数据集。这些晶体在光子工厂的KEK-NW12光束线上衍射到最高分辨率2.8 Å，并给出了一个总体Rsym为7.9%、完整性为91%的数据集。正在尝试通过SIRAS方法解析其结构。
