Nakano T
Department of Molecular Cell Biology, Osaka University, Japan.
Int J Hematol. 1996 Dec;65(1):1-8. doi: 10.1016/s0925-5710(96)00531-2.
We developed an efficient differentiation induction system (OP9 system) from mouse embryonic stem (ES) cell to blood cells by coculture on a novel stromal cell line, OP9. This stromal cell line does not produce functional macrophage colony-stimulating factor (M-CSF) and presumably the deficiency of M-CSF production of OP9 cells might induce preferential differentiation of ES cells into hematopoietic cells other than the monocyte-macrophage lineage. ES cells gave rise to embryonic primitive erythrocytes, and then adult type definitive erythrocytes, myeloid and B lineage cells. The OP9 system should facilitate to elucidate the molecular mechanisms of hematopoietic system development and hematopoietic cell differentiation.
我们通过在一种新型基质细胞系OP9上共培养，开发了一种从小鼠胚胎干细胞高效诱导分化为血细胞的系统（OP9系统）。这种基质细胞系不产生功能性巨噬细胞集落刺激因子（M-CSF），推测OP9细胞M-CSF产生的缺乏可能诱导胚胎干细胞优先分化为单核细胞-巨噬细胞谱系以外的造血细胞。胚胎干细胞产生胚胎原始红细胞，然后产生成年型定型红细胞、髓系细胞和B淋巴细胞系细胞。OP9系统应有助于阐明造血系统发育和造血细胞分化的分子机制。
