Brodskiĭ V Ia, Nechaeva N V, Terskikh V V, Novikova T E, Gvazava I G, Fateeva V I
Izv Akad Nauk Ser Biol. 1996 Jul-Aug(4):398-401.
In a culture of rat hepatocytes, kept in a serum-free medium 199, the number of attached cells was decreased and a monolayer was not formed within 24 h. Soon after the cells were attached to collagen, the intensity of protein synthesis in the serum-free medium was decreased due to the smaller number of cells in the culture and was calculated per cell. The properties of cultures, i.e., the number and shape of cells, external appearance of the culture, and intensity of protein synthesis, were similar in a serum-containing medium and in the serum-free medium 199 complemented with 0.2 mg/ml albumin and 5 micrograms/ml insulin. A monolayer typical for the medium with serum was formed in the medium with albumin and insulin within approximately 20 h after attachment of the hepatocytes to the substrate.
在无血清培养基199中培养的大鼠肝细胞中，贴壁细胞数量减少，24小时内未形成单层。细胞附着于胶原蛋白后不久，由于培养物中细胞数量较少，无血清培养基中蛋白质合成强度降低，并按每个细胞计算。在含血清培养基以及添加了0.2mg/ml白蛋白和5μg/ml胰岛素的无血清培养基199中，培养物的特性，即细胞数量和形状、培养物外观以及蛋白质合成强度相似。在肝细胞附着于底物后约20小时，含白蛋白和胰岛素的培养基中形成了典型的含血清培养基单层。
