利用杆状病毒表达的E1蛋白检测风疹病毒特异性免疫球蛋白M抗体
Detection of rubella virus-specific immunoglobulin M antibodies with a baculovirus-expressed E1 protein.
作者信息
Schmidt M, Lindqvist C, Salmi A, Oker-Blom C
机构信息
Department of Biochemistry and Pharmacy, Abo Akademi University, Turku, Finland.
出版信息
Clin Diagn Lab Immunol. 1996 Mar;3(2):216-8. doi: 10.1128/cdli.3.2.216-218.1996.
Abstract
The structural proteins of rubella virus (RV) were expressed in insect cells by using the baculovirus expression vector system. The recombinant E1 envelope glycoprotein was purified by immunoaffinity chromatography and used to detect RV-specific immunoglobulin M antibodies in a time-resolved fluoroimmunoassay. Correlation analysis between the reactivities of antibodies against this recombinant E1 and the reactivities against authentic RV antigen shows that purified E1 can detect RV antibodies of the immunoglobulin M type.
摘要
通过杆状病毒表达载体系统在昆虫细胞中表达风疹病毒（RV）的结构蛋白。重组E1包膜糖蛋白通过免疫亲和层析纯化，并用于时间分辨荧光免疫分析中检测RV特异性免疫球蛋白M抗体。针对这种重组E1的抗体反应性与针对天然RV抗原的反应性之间的相关性分析表明，纯化的E1可以检测免疫球蛋白M型的RV抗体。
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