Richards D T, Hoole D, Lewis J W, Ewens E, Arme C
Centre for Applied Entomology & Parasitology, Department of Biological Sciences, Keele University, UK.
J Helminthol. 1996 Mar;70(1):63-7. doi: 10.1017/s0022149x00015145.
Live adult and cercarial stages of Sanguinicola inermis Plehn, 1905 (Trematoda:Sanguinicolidae) were maintained in vitro in the presence of carp (Cyprinus carpio L.) leucocytes. Cells and parasites were fixed at intervals from 0.25 to 48 h and examined using light microscopy, SEM and TEM. Within 12 h of exposure, leucocytes were found attached to cercariae although, by 24 h, fewer cells were found attached to postcercarial, juvenile adult stages that had shed their tails. Neutrophils and macrophages were found attached to the damaged tegument of cercarie that had not transformed by 48 h. Few cells were attached to the tegument of adult flukes that were alive when fixed. However, there was extensive tegumental damage and numerous cells were attached to adult flukes that had died before fixation. The results are discussed with reference to parasite survival within the vascular system of the host.
1905年的无鳍血居吸虫（吸虫纲：血居科）的成虫和尾蚴阶段在鲤鱼（鲤属鲤）白细胞存在的情况下进行体外培养。细胞和寄生虫在0.25至48小时的间隔内固定，并使用光学显微镜、扫描电子显微镜和透射电子显微镜进行检查。在暴露的12小时内，发现白细胞附着在尾蚴上，尽管到24小时时，发现附着在已脱掉尾巴的后尾蚴、幼年成虫阶段的细胞较少。发现中性粒细胞和巨噬细胞附着在48小时内未转化的尾蚴受损的体表上。固定时存活的成虫吸虫体表附着的细胞很少。然而，有广泛的体表损伤，许多细胞附着在固定前死亡的成虫吸虫上。结合寄生虫在宿主体内血管系统中的存活情况对结果进行了讨论。
