Nguyen N X, Martus P, Küchle M
Augenklinik mit Poliklinik, Universität Erlangen-Nürnberg.
Klin Monbl Augenheilkd. 1996 Aug-Sep;209(2-3):89-93. doi: 10.1055/s-2008-1035284.
The laser flare meter FM-500 (Kowa, diode laser, 670 nm) is a simplified version of the laser flare-cell meter FC-1000 (Kowa, He-Ne laser, 632.8 nm). the aim of this study was to compare flare measurements of the laser flare meter FC-500 with the established laser flare-cell meter FC-1000 and to determine the reproducibility of both instruments in vivo.
Ten eyes of 10 normal subjects (mean age 32.2 +/- 8.0 y) and 21 eyes of 21 patients (mean age 36.7 +/- 17.2 y) with various forms of uveitis were included in the study. Aqueous flare was quantified using the laser flare-cell meter FC-1000 and the laser flare meter FM-500 by one examiner under comparable conditions. The selection of eyes as well as the order of the examinations were done in a randomized fashion. Measurements with both instruments were repeated the following day in normal eyes. Statistical analysis was done using linear regression analysis, intraclass correlation and U-test.
Flare values (photon counts/ms) did not differ significantly between the instruments either for normal eyes (3.3 +/- 0.4 vs 3.5 +/- 0.6) or for eyes with uveitis (16.2 +/- 9.0 vs 16.4 +/- 8.7). A high correlation of flare counts between both instruments was observed in normal eyes (r = 0.73, p < 0.001) and a very high correlation in eyes (R = 0.99, p < 0.0001) with mildly to moderately increased counts (< 50 photon counts/ms). Differences between flare values of both instruments were -0.17 +/- 0.4, range -0.8 +/- 0.6 in the normal eyes and -0.23 +/- 1.38, range -3.1 +/- 3.4 in the patients with mildly to moderately increased flare counts. However, flare counts differed for very high aqueous flare in one patient with acute fibrinous iridocyclitis with the FM-500 indicating higher values. The intraclass correlation coefficients were 0.70 for the FC-1000 and 0.76 for the FM-500 in normal eyes and 0.99 for both instruments in eyes with uveitis.
In eyes without or with mild to moderate breakdown of the blood-aqueous barrier, the two instruments give comparable results. However, measurements may differ in eyes with high flare readings.
激光闪光细胞仪FM - 500（拓普康，二极管激光器，670纳米）是激光闪光细胞仪FC - 1000（拓普康，氦氖激光器，632.8纳米）的简化版本。本研究的目的是比较激光闪光细胞仪FM - 500与已有的激光闪光细胞仪FC - 1000的闪光测量结果，并确定这两种仪器在体内的可重复性。
本研究纳入了10名正常受试者的10只眼（平均年龄32.2±8.0岁）和21名患有各种葡萄膜炎的患者的21只眼（平均年龄36.7±17.2岁）。由一名检查者在可比条件下使用激光闪光细胞仪FC - 1000和激光闪光细胞仪FM - 500对房水闪光进行定量。眼睛的选择以及检查顺序均采用随机方式。正常眼在次日重复使用两种仪器进行测量。采用线性回归分析、组内相关分析和U检验进行统计分析。
无论是正常眼（3.3±0.4对3.5±0.6）还是葡萄膜炎眼（16.2±9.0对16.4±8.7），两种仪器的闪光值（光子计数/毫秒）均无显著差异。在正常眼中观察到两种仪器的闪光计数高度相关（r = 0.73，p < 0.001），在闪光计数轻度至中度增加（<50光子计数/毫秒）的眼中相关性非常高（R = 0.99，p < 0.0001）。正常眼中两种仪器闪光值的差异为-0.17±0.4，范围为-0.8±0.6；闪光计数轻度至中度增加的患者中差异为-0.23±1.38，范围为-3.1±3.4。然而，一名急性纤维素性虹膜睫状体炎患者的房水闪光值极高时，FM - 500测量的闪光计数较高，两种仪器的测量结果不同。正常眼中FC - 1000的组内相关系数为0.70，FM - 500为0.76；葡萄膜炎眼中两种仪器的组内相关系数均为0.99。
在血-房水屏障未破坏或轻度至中度破坏的眼中，两种仪器的测量结果相当。然而，在闪光读数较高的眼中，测量结果可能不同。
