Petersen I, Schwendel A, Bockmühl U, Dietel M
Institut für Pathologie der Charité, Humboldt-Universität, Berlin.
Pathologe. 1996 Sep;17(5):333-41. doi: 10.1007/s002920050170.
Comparative genomic hybridization (CGH) is a new method of screening a tumor for genetic changes. The alterations are classified as DNA gains and losses and reveal a characteristic pattern that includes mutations at the chromosomal and subchromosomal levels. Although DNA from fresh-frozen tissue is recommended as the starting material, archival specimens can also be analyzed. Various examples are presented that illustrate the changes that occur in different tumor entities, and preneoplastic lesions, the differentiation of primary tumors from metastases, and the investigation of tumor cell lines that are resistant to cytostatic drugs. These examples emphasize that CGH can extend the possibilities for genetic diagnosis in tumor pathology.
比较基因组杂交（CGH）是一种用于筛查肿瘤基因变化的新方法。这些改变被分类为DNA的增加和缺失，并揭示出一种特征性模式，其中包括染色体和亚染色体水平的突变。虽然建议使用新鲜冷冻组织的DNA作为起始材料，但存档标本也可进行分析。文中给出了各种实例，阐述了不同肿瘤实体、癌前病变中发生的变化，原发性肿瘤与转移瘤的鉴别，以及对细胞毒性药物耐药的肿瘤细胞系的研究。这些实例强调，CGH可以拓展肿瘤病理学基因诊断的可能性。
