Roth K, Wolf G, Dietel M, Petersen I
Institut für Pathologie der Charité, Medizinische Fakultät der Humboldt-Universität zu Berlin.
Pathologe. 1996 Sep;17(5):342-8. doi: 10.1007/s002920050171.
Comparative genomic hybridization (CGH) is a molecular cytogenetic method for the detection of chromosomal imbalances between a tumor and a normal genome. In order to produce quantitative and reproducible results, we developed an image analysis program that allows the detection and mapping of the genetic alterations. The result is represented as a CGH sum karyogram in which the genetic changes are documented as color coded-chromosomes. The aim of our investigation is to correlate the genotype with the phenotype on the basis of CGH sum karyograms and thus to achieve a genetic characterization that will supplement the morphological tumor description.
比较基因组杂交（CGH）是一种用于检测肿瘤基因组与正常基因组之间染色体失衡的分子细胞遗传学方法。为了产生定量且可重复的结果，我们开发了一种图像分析程序，该程序可检测并绘制基因改变图谱。结果以CGH总和核型图表示，其中基因变化记录为颜色编码的染色体。我们研究的目的是根据CGH总和核型图将基因型与表型相关联，从而实现一种基因特征描述，以补充肿瘤的形态学描述。
