Cubel R C, Siqueira M M, Santos E O, Pires M F, Cruz C M, Nascimento J P
Departamento de Virologia da Fundação Oswaldo Cruz, Rio de Janeiro, Brasil.
Rev Soc Bras Med Trop. 1997 Jan-Feb;30(1):15-20. doi: 10.1590/s0037-86821997000100004.
A total of 1397 sera collected from 1095 cases of exanthematic disease notified as measles in ES and RJ states during July 1992 to December 1994 were investigated. These sera were first tested for measles and rubella specific IgM. When they proved negative, they were tested for B19 specific IgM by an enzyme immunoassay. B19 infection was confirmed in 27 (2.5%) of these cases. Sera from 194 negative cases for measles and rubella IgM received from other Brazilian states were also investigated and B19 infection was confirmed for 11 of them. Sera from these 38 IgM positive cases for B19, were tested for anti-B19 IgG by an enzyme immunoassay and for B19 DNA by dot blot hybridization. Anti-B19 IgG antibodies were detected in most of the acute sera. B19 DNA was detected in the acute serum of one patient that had been splenectomized before. As the exanthem caused by human parvovirus infection may be clinically diagnosed as rubella, it could be important to diagnose B19 infection in Brazil since it is becoming prevalent as the cause of rash in countries where rubella is controlled by vaccination.
对1992年7月至1994年12月期间在圣保罗州和里约热内卢州报告为麻疹的1095例发疹性疾病患者采集的1397份血清进行了调查。这些血清首先检测麻疹和风疹特异性IgM。当检测结果为阴性时，通过酶免疫测定法检测B19特异性IgM。在这些病例中，27例（2.5%）确诊为B19感染。还对从巴西其他州收到的194例麻疹和风疹IgM检测阴性病例的血清进行了调查，其中11例确诊为B19感染。对这38例B19 IgM阳性病例的血清通过酶免疫测定法检测抗B19 IgG，并通过斑点杂交法检测B19 DNA。在大多数急性期血清中检测到抗B19 IgG抗体。在一名既往接受过脾切除术患者的急性期血清中检测到B19 DNA。由于人细小病毒感染引起的皮疹在临床上可能被诊断为风疹，鉴于在风疹通过疫苗接种得到控制的国家，B19作为皮疹病因正变得越来越普遍，因此在巴西诊断B19感染可能很重要。
