Nikitina S M, Savchenko O N, Kogan M E, Ezhkova N S
Zh Evol Biokhim Fiziol. 1977 Jul-Aug;13(4):443-7.
Extraction and quantitative determination of progesterone, testosterone and oestrogens have been made in tissues of cephalopod molluscs and crustaceans. Extraction was made by acetone and ethyl ether. Purification was performed by thin layer chromatography on aluminium oxide and silicagel in different systems. The quantitative assay of progesterone and oestrogens was made by fluorimetric and radioimmunological techniques, that of testosterone--by spectrophotometric determination of hydrozones and radioimmunological procedure. Progesterone and testosterone were obtained from the gonads and liver of cephalopods and from the whole body of crustaceans. During sexual maturation of the animals concentration of these hormones tends to increase. Oestrone and oestradiol were found only in the gonads of cephalopods.
已对头足类软体动物和甲壳类动物的组织进行了孕酮、睾酮和雌激素的提取及定量测定。采用丙酮和乙醚进行提取。通过在不同体系中于氧化铝和硅胶上进行薄层层析来实现纯化。孕酮和雌激素的定量测定采用荧光法和放射免疫技术，睾酮的定量测定则通过对腙的分光光度法测定及放射免疫程序进行。孕酮和睾酮取自头足类动物的性腺和肝脏以及甲壳类动物的整体。在动物的性成熟过程中，这些激素的浓度趋于增加。仅在头足类动物的性腺中发现了雌酮和雌二醇。
