Solano P, Reifenberg J M, Amsler-Delafosse S, Kabore I, Cuisance D, Duvallet G
CIRDES, Bobo-Dioulasso, Burkina Faso.
Med Vet Entomol. 1996 Oct;10(4):354-8. doi: 10.1111/j.1365-2915.1996.tb00756.x.
Following the discovery of four cases of African human trypanosomiasis, an entomological survey was conducted along the Mouhoun river in southwest Burkina Faso to collect Glossina palpalis gambiensis and G.tachinoides. Among 226 flies dissected, 4.87% (eleven individuals) were infected in midgut or proboscis, but never in the salivary glands. Polymerase chain reaction analysis was undertaken, and was able to characterize all the proboscis infections, and half of the midgut infections. Only Trypanosoma simiae and T. vivax were found in the organs of infected flies, in single or mixed-species infections. Ten more flies, negative with parasitological examination, were tested with Trypanozoon primers and remained negative. The epidemiological significance of the absence of T.brucei group infections in wild tsetse populations and the presence of T.simiae in G.p.gambiensis are discussed.
在发现4例非洲人类锥虫病病例后，在布基纳法索西南部的穆洪河沿岸开展了一项昆虫学调查，以采集冈比亚须舌蝇和嗜人舌蝇。在解剖的226只苍蝇中，4.87%（11只个体）的中肠或喙部受到感染，但唾液腺从未受到感染。进行了聚合酶链反应分析，能够鉴定出所有喙部感染以及一半的中肠感染。在受感染苍蝇的器官中，仅发现了西氏锥虫和活泼锥虫，存在单物种感染或混合物种感染。另外10只经寄生虫学检查呈阴性的苍蝇，用布氏锥虫引物进行检测，结果仍为阴性。本文讨论了野生采采蝇种群中未出现布氏锥虫属感染以及冈比亚须舌蝇中存在西氏锥虫的流行病学意义。
