Kuschinsky W
Department of Physiology, University of Heidelberg, Germany.
Pflugers Arch. 1996;432(3 Suppl):R42-6.
The density of the total and the perfused capillary network was determined in the brains of awake, normocapnic rats. Perfused capillaries were marked by i.v. Evans blue. Existing capillaries were made visible by antibodies directed against the fibronectin portion of the capillary walls. Comparison of perfused and existing capillaries in identical brain sections by double staining showed a high degree of congruence between perfused and existing brain capillaries. The results show a continuous perfusion of all capillaries in the brain of the awake normocapnic rat. In the second group of experiments capillary perfusion was investigated during a reduction of cerebral blood flow induced by incomplete forebrain ischaemia. These experiments showed a gross preservation of capillary flow in the brain capillaries, although the velocity of perfusion was reduced. Finally, a method was developed which allows the distribution pattern of brain capillary plasma flow to be examined. The third group of experiments which used this method indicates a heterogeneous distribution of capillary flow in the brain under normocapnic conditions and a reduction of heterogeneity during hypocapnic hyperaemia.
在清醒、正常碳酸血症大鼠的大脑中测定了总毛细血管网络和灌注毛细血管网络的密度。通过静脉注射伊文思蓝标记灌注的毛细血管。利用针对毛细血管壁纤连蛋白部分的抗体使现存毛细血管可见。通过双重染色对相同脑切片中的灌注毛细血管和现存毛细血管进行比较，结果显示灌注的脑毛细血管和现存的脑毛细血管高度一致。结果表明，清醒正常碳酸血症大鼠大脑中的所有毛细血管都持续灌注。在第二组实验中，研究了在前脑不完全缺血诱导的脑血流量减少期间的毛细血管灌注情况。这些实验表明，尽管灌注速度降低，但脑毛细血管中的血流仍大体保持。最后，开发了一种方法，可用于检查脑毛细血管血浆流动的分布模式。使用该方法的第三组实验表明，在正常碳酸血症条件下，脑内毛细血管血流分布不均，而在低碳酸血症性充血期间不均一性降低。
