Theilen H, Schröck H, Kuschinsky W
Department of Physiology, University of Heidelberg, Germany.
J Cereb Blood Flow Metab. 1994 Nov;14(6):1055-61. doi: 10.1038/jcbfm.1994.138.
The densities of perfused and existing capillaries were measured in different cortical regions of rat brains after subtemporal occlusion of the middle cerebral artery (MCA). Capillary perfusion patterns (perfused capillaries versus nonperfused capillaries) were verified immediately after MCA occlusion in one group of rats and 1 h later in a second group using fluorescent double staining of capillary morphology and plasma perfusion in identical brain sections. In addition, local cerebral blood flow (CBF) was measured in another group of rats 1 h after MCA occlusion using the autoradiographic iodo-[14C]-antipyrine method. Although cortical CBF was decreased by up to 75% 1 h after MCA occlusion, plasma perfusion was not completely stopped in most capillaries (circulation time of Evans blue, 10 s). Only small patchy perfusion deficits (< 0.1 mm2 of brain section) were detected in the capillaries immediately and 1 h after MCA occlusion in all brains except for one that exhibited a more extensive lack of capillary perfusion. The data show that a drastic reduction of cortical CBF after MCA occlusion is not accompanied by a corresponding amount of nonperfused capillaries.
在大脑中动脉（MCA）颞下闭塞后，测量大鼠大脑不同皮质区域灌注毛细血管和现存毛细血管的密度。在一组大鼠中，MCA闭塞后立即使用荧光双染法对毛细血管形态和血浆灌注进行相同脑切片染色，以验证毛细血管灌注模式（灌注毛细血管与未灌注毛细血管）；在另一组大鼠中，1小时后进行验证。此外，在另一组大鼠中，MCA闭塞1小时后，使用放射自显影碘代-[14C]-安替比林法测量局部脑血流量（CBF）。尽管MCA闭塞1小时后皮质CBF降低了75%，但大多数毛细血管中的血浆灌注并未完全停止（伊文思蓝循环时间为10秒）。除了一个表现出更广泛毛细血管灌注缺乏的大脑外，在所有大脑中，MCA闭塞后立即和1小时后，在毛细血管中仅检测到小片状灌注缺损（脑切片面积<0.1平方毫米）。数据表明，MCA闭塞后皮质CBF的急剧降低并未伴随着相应数量的未灌注毛细血管。
