Eaton M C, Hardingham J E, Kotasek D, Dobrovic A
University of Adelaide, Queen Elizabeth Hospital, Woodville, Australia.
Biotechniques. 1997 Jan;22(1):100-5. doi: 10.2144/97221st01.
Detection of circulating tumor cells and micrometastases in patients with cancer should prove useful in determining prognosis and in planning and monitoring systemic therapies. We have developed immunomagnetic isolation of carcinoma cells followed by reverse transcription polymerase chain reaction (immunobead RT-PCR) as a method for identifying very small numbers of breast cancer cells in blood. The expression of cytokeratin 19 (K19) was used as the marker by which the isolated tumor cells were identified. The immunobead RT-PCR technique allowed detection of one tumor cell per 10(6) leukocytes in whole blood. Immunobead RT-PCR is a highly sensitive method of detecting cancer cells in a hematopoietic environment.
检测癌症患者循环肿瘤细胞和微转移对于判断预后以及规划和监测全身治疗应具有重要意义。我们已经开发出一种免疫磁珠分离癌细胞，随后进行逆转录聚合酶链反应（免疫磁珠RT-PCR）的方法，用于识别血液中极少量的乳腺癌细胞。细胞角蛋白19（K19）的表达被用作鉴定分离出的肿瘤细胞的标志物。免疫磁珠RT-PCR技术能够检测全血中每10⁶个白细胞中的一个肿瘤细胞。免疫磁珠RT-PCR是在造血环境中检测癌细胞的一种高度灵敏的方法。
