Marracci S, Batistoni R, Pesole G, Citti L, Nardi I
Laboratori di Biologia Cellulare e dello Sviluppo, Dipartimento di Fisiologia e Biochimica, Pisa, Italy.
J Mol Evol. 1996 Dec;43(6):584-93. doi: 10.1007/BF02202106.
We have studied a family of long repetitive DNA sequences (Hsr1) interspersed in the large genome of the European plethodontid salamander Hydromantes. The sequence analysis of a 5-kb fragment (Hsr1A) of one member has revealed significant similarities with amino acidic domains of retroviruses and retrotransposons. The similarity of the reverse transcriptase domain and the gene organization identifies Hsr1A as a member of the gypsy/Ty3 class of retrotransposons. We hypothesize that Hsr1 sequences are vestiges of an invasion of the Hydromantes genome that occurred early in the evolutionary history of these European plethodontids. About 10(6) Hsr1 sequences are present in the large Hydromantes genome. This is the highest number of copies so far discovered for retrotransposon-like elements in eukaryote organisms.
我们研究了一族散布在欧洲无肺螈科蝾螈Hydromantes大基因组中的长重复DNA序列（Hsr1）。对其中一个成员的5千碱基片段（Hsr1A）进行的序列分析显示，它与逆转录病毒和逆转座子的氨基酸结构域存在显著相似性。逆转录酶结构域的相似性及基因组织表明Hsr1A属于逆转座子的gypsy/Ty3类成员。我们推测，Hsr1序列是在这些欧洲无肺螈进化历史早期发生的Hydromantes基因组入侵的遗迹。在庞大的Hydromantes基因组中存在约10^6个Hsr1序列。这是迄今为止在真核生物中发现的逆转座子样元件的最高拷贝数。
