Beran J, Douda P, Prymula R, Gál P, Rychlý R, Splino M
Purkynĕ Military Medical Academy, Department of Epidemiology, Hradec Králové, Czech Republic.
Cent Eur J Public Health. 1996 May;4(2):87-90.
Viral hepatitis A is a common disease, particularly in developing countries. All staff and troops of the U.N. (United Nations) are vaccinated by the Havrix vaccine. Till 1995 we did not vaccinate Czech troops which have operated in the area of former Yugoslavia. The main goal of this study was to obtain data about the seroprevalence of hepatitis A antibody among the Czech U.N. troops before their departure to the conflict area and to optimize the vaccination approach. The serum samples were examined by the MEIA (Microparticle Enzyme Immunoassay) method in the fully automated system for immunoassays IMx in the Military Institute of Health in Ceské Budĕjovice. We used HAVAB kits of Abbott Company. 692 serum samples (military staff of the Czech U.N. troops in Yugoslavia) were examined in 1991-1995. In the laboratory 19 samples were eliminated due to small amounts (less than 50 microliters) or haemolysis. 673 (1991 - 65, 1992 - 296, 1993 - 265, 1994 - 35, 1995 - 12) were investigated. The staff was divided into four age cohorts by decades (20-29, 30-39, 40-49, 50-59); 26.0%, 47.4%, 24.9% and 1.7%, respectively. There were 253 (37.4%) positive samples and 420 (62.6%) negatives ones, ratio 1:1.7. The ratio of the positive and negative samples--immunity rate and seroprevalence--were 1:0.4 (26.2%), 1:0.6 (37.9%); 1:0.9 (46.4%) and 1:2.7 (72.7%), respectively in the age cohorts. The results show a relatively low seroprevalence of the anti-HAV antibody in all the age cohorts and necessity to vaccinate the Czech U.N. troops by the special basic schedule--Havrix 2 x 720 E.U. at the same time. This regimen will be used in the new units that will be stationed in Bosnia. So far 200 persons have been vaccinated in this way.
甲型病毒性肝炎是一种常见疾病，在发展中国家尤为如此。联合国所有工作人员和部队都接种了甲肝疫苗（Havrix）。直到1995年，我们都没有为在前南斯拉夫地区执行任务的捷克部队接种疫苗。本研究的主要目的是获取捷克联合国部队在前往冲突地区之前甲型肝炎抗体血清流行率的数据，并优化疫苗接种方法。血清样本在捷克布杰约维采军事卫生研究所的全自动免疫分析系统IMx中采用微粒子酶免疫分析法（MEIA）进行检测。我们使用的是雅培公司的HAVAB试剂盒。1991年至1995年期间对692份血清样本（驻南斯拉夫的捷克联合国部队军事人员）进行了检测。在实验室中，19份样本因量少（少于50微升）或溶血而被排除。对673份样本（1991年 - 65份，1992年 - 296份，1993年 - 265份，1994年 - 35份，1995年 - 12份）进行了调查。工作人员按年龄段分为四个组（20 - 29岁、30 - 39岁、40 - 49岁、50 - 59岁），各年龄段的比例分别为26.0%、47.4%、24.9%和1.7%。有253份（37.4%）阳性样本和420份（62.6%）阴性样本，比例为1:1.7。在各年龄组中，阳性和阴性样本的比例——免疫率和血清流行率——分别为1:0.4（26.2%）、1:0.6（37.9%）、1:0.9（46.4%）和1:2.7（72.7%）。结果显示，所有年龄组中抗甲型肝炎病毒抗体的血清流行率相对较低，有必要按照特殊的基础接种程序——同时接种两剂720欧洲单位的甲肝疫苗（Havrix），为捷克联合国部队进行接种。这一接种方案将用于即将驻扎在波斯尼亚的新部队。到目前为止，已有200人按此方式接种了疫苗。
