将DNA的结构分析与用于检测转录调控元件的搜索程序相结合。
Combining structural analysis of DNA with search routines for the detection of transcription regulatory elements.
作者信息
Karas H, Knüppel R, Schulz W, Sklenar H, Wingender E
机构信息
Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.
出版信息
Comput Appl Biosci. 1996 Oct;12(5):441-6. doi: 10.1093/bioinformatics/12.5.441.
MOTIVATION
Analysis of unannotated genomic sequences for regulatory regions depends on a reliable recognition of individual cis-acting elements. Since some of them have a very low conserved sequence pattern, additional criteria are required.
RESULTS
Using molecular modelling techniques, we have created a complete database for the conversion of base sequences into profiles of structural parameters of DNA. On this basis, search routines can be developed that scan for profile matches. They may be used instead of or, probably most appropriate in most cases, in combination with conventional sequence pattern searches.
动机
对未注释的基因组序列进行调控区域分析依赖于对单个顺式作用元件的可靠识别。由于其中一些元件具有非常低的保守序列模式，因此需要额外的标准。
结果
使用分子建模技术，我们创建了一个完整的数据库，用于将碱基序列转换为DNA结构参数概况。在此基础上，可以开发搜索程序来扫描概况匹配。它们可以替代传统的序列模式搜索，或者在大多数情况下可能最合适的是与传统序列模式搜索结合使用。
Zotero 插件