Garwicz M, Apps R, Trott J R
Department of Physiology, School of Medical Sciences, University of Bristol, UK.
Eur J Neurosci. 1996 Dec;8(12):2726-38. doi: 10.1111/j.1460-9568.1996.tb01567.x.
The olivocerebellar and corticonuclear connections of the forelimb area of the paravermal medial C3 zone were studied in the cat using a combined electrophysiological and fluorescent tracer technique. During an initial operation under barbiturate anaesthesia, lobules IV/V of the cerebellar anterior lobe were exposed and small injections of dextran amines tagged with rhodamine or fluorescein were made into areas selected from four different electrophysiologically defined parts of the zone. The inferior olive and the deep cerebellar nuclei were then scrutinized for retrogradely labelled cells and anterogradely labelled axon terminals respectively. The findings demonstrate a detailed topographical organization within the olivocerebellar projection to the medial C3 zone and provide some evidence for a topographical organization of its projection to nucleus interpositus anterior. Both projections are described at a level of resolution not previously attained in neuroanatomical studies and the results strongly support the notion of a micro-compartmentalization of cerebellar olivo-corticonuclear circuits.
运用电生理和荧光示踪剂相结合的技术，对猫的小脑蚓旁内侧C3区前肢代表区的橄榄小脑和皮质延髓联系进行了研究。在巴比妥类麻醉下的初次手术过程中，暴露小脑前叶的IV/V小叶，并将用罗丹明或荧光素标记的葡聚糖胺微量注射到该区域四个不同电生理界定部位中的选定区域。随后分别在橄榄下核和小脑深部核团中仔细寻找逆行标记细胞和顺行标记轴突终末。研究结果显示了橄榄小脑向内侧C3区投射的详细拓扑组织，并为其向前庭间核投射的拓扑组织提供了一些证据。这两种投射均在神经解剖学研究中未曾达到的分辨率水平上进行了描述，结果有力地支持了小脑橄榄 - 皮质延髓回路存在微区室化的观点。
