Utsumi H, Tano K, Mizuma N, Kobayashi T, Ichihashi M
Research Reactor Institute, Kyoto University, Osaka, Japan.
J Radiat Res. 1996 Sep;37(3):193-8. doi: 10.1269/jrr.37.193.
We studied the effect of neutron capture treatment using 10B-compound on X-ray sensitive P-39 and X-ray resistant G-361 human melanoma cell lines, and found a high lethal effect of boron neutron capture therapy in comparison with conventional ionizing radiation. The P-39 line was sensitive to thermal neutron radiation, and extremely sensitive to bleomycin treatment, whereas the G-361 line was resistant to both forms of treatment; however, the two cell lines had similar sensitivity to thermal neutron radiation after pretreatment with 10B1-para-boronophenylalanine (10B1-BPA, 200 micrograms/ml medium). These results show that the thermal neutron capture products (a 7Li nucleus and alpha particle) are highly damaging and short range in tumor cells and thus more efficiently inactivate melanoma cells irrespective of x-ray sensitivity, than conventional X-ray-irradiation.
我们研究了使用含硼化合物进行中子俘获治疗对X射线敏感的P - 39和X射线抗性的G - 361人黑色素瘤细胞系的影响，发现与传统电离辐射相比，硼中子俘获疗法具有很高的致死效应。P - 39细胞系对热中子辐射敏感，对博来霉素治疗极其敏感，而G - 361细胞系对这两种治疗方式均有抗性；然而，在用10B1 - 对硼苯丙氨酸（10B1 - BPA，200微克/毫升培养基）预处理后，这两种细胞系对热中子辐射具有相似的敏感性。这些结果表明，热中子俘获产物（一个锂 - 7核和α粒子）在肿瘤细胞中具有高度破坏性且射程短，因此与传统X射线照射相比，无论X射线敏感性如何，都能更有效地使黑色素瘤细胞失活。
