Weng X, Cloutier G, Beaulieu R, Roederer G O
Laboratory of Biomedical Engineering, Hôtel-Dieu of Montreal Hospital, Quebec, Canada.
Am J Physiol. 1996 Dec;271(6 Pt 2):H2346-52. doi: 10.1152/ajpheart.1996.271.6.H2346.
With the exception of fibrinogen, immunoglobulins, and albumin, little information is available on the effect of acute-phase proteins on erythrocyte aggregation. The objective of this study was to investigate the effects of haptoglobin (Hp), C-reactive protein (CRP), ceruloplasmin (Cp), alpha 1-acid glycoprotein (alpha 1-AGP), and alpha 1-antitrypsin (alpha 1-AT) on the aggregation kinetics and shear resistance of erythrocyte aggregates. The plasma concentration of these proteins was measured in 20 healthy individuals and kept unchanged while the concentration of the protein tested was increased. Adding Hp to concentrations between 2.78 and 4.99 g/l resulted in a significant progressive increase in aggregation kinetics compared with controls. An elevation of the shear resistance of the aggregates was found for CRP at a concentration of 0.438 g/l. By an increase in the concentration of Cp from 4.40 to 9.39 g/l, the aggregation kinetics and the adhesive forces between erythrocytes were significantly increased: No effect on erythrocyte aggregation was observed for alpha 1-AGP, alpha 1-AT, and Cp at concentrations of 2.85, 3.97, and 2.43 g/l, respectively. The molecular mass of the acute-phase proteins, their configuration, and the presence of specific receptors on the erythrocyte membrane are postulated as possible factors influencing erythrocyte aggregation.
除纤维蛋白原、免疫球蛋白和白蛋白外，关于急性期蛋白对红细胞聚集的影响，目前所知甚少。本研究的目的是探讨触珠蛋白（Hp）、C反应蛋白（CRP）、铜蓝蛋白（Cp）、α1-酸性糖蛋白（α1-AGP）和α1-抗胰蛋白酶（α1-AT）对红细胞聚集体聚集动力学和抗剪切力的影响。在20名健康个体中测定了这些蛋白的血浆浓度，并保持不变，同时增加被测蛋白的浓度。与对照组相比，将Hp添加到浓度在2.78至4.99 g/l之间会导致聚集动力学显著逐步增加。发现当CRP浓度为0.438 g/l时，聚集体的抗剪切力有所提高。将Cp浓度从4.40 g/l增加到9.39 g/l时，聚集动力学和红细胞之间的粘附力显著增加：分别在2.85、3.97和2.43 g/l的浓度下，未观察到α1-AGP、α1-AT和Cp对红细胞聚集有影响。急性期蛋白的分子量、其构型以及红细胞膜上特定受体的存在被认为是影响红细胞聚集的可能因素。
