Weng X, Roederer G O, Beaulieu R, Cloutier G
Laboratory of Biomedical Engineering, Clinical Research Institute of Montréal, Québec, Canada.
Thromb Haemost. 1998 Dec;80(6):903-8.
Numerous studies have demonstrated that elevated concentrations of acute-phase proteins affect red blood cell (RBC) aggregation. Plasma lipids and lipoproteins were also shown to be correlated with RBC aggregation in hypercholesterolemia. However, whether acute-phase proteins promote RBC hyperaggregation in hyperlipidemic patients is unknown. The main objective of the study was to identify the impact of acute-phase proteins such as fibrinogen (Fib), haptoglobin (Hp), ceruloplasmin (Cp), alpha1-acid glycoprotein (AGP), alpha1-antitrypsin (AT), immunoglobulin G (IgG), and albumin (Alb) on RBC aggregation in 35 hyperlipidemic patients. The influence of these proteins in 32 normolipidemic subjects was also determined.
RBC aggregation parameters reflecting the kinetics of rouleau formation and the adhesive strength between RBCs were measured by laser reflectometry. Multivariate forward stepwise linear regression analyses were performed to study the relationship between RBC aggregation and these acute-phase proteins, total cholesterol (TC), triglycerides (TG), high (HDL-C) and low (LDL-C) density lipoprotein cholesterol, age, gender, body mass index (BMI), mean blood pressure (Mpresure), and smoking habit. The kinetics of rouleau formation was positively correlated with the linear combination of IgG and Hp (r=0.76, p <0.0001) in hyperlipidemic patients, whereas IgG, smoking, AGP and gender were significant independent predictors in healthy subjects (r=0.79, p <0.0001). The correlations obtained for the models predicting the adhesive strength between RBCs were 0.69 in patients (Alb, HDL-C, IgG, p <0.002) and 0.71 in healthy individuals (AGP, BMI, p <0.0001).
This study suggests that acute-phase proteins such as IgG, Hp, AGP and Alb influence significantly and in an independent way the level of RBC aggregation. The close association between RBC aggregation and cardiovascular risk factors further strengthens its clinical importance.
大量研究表明，急性期蛋白浓度升高会影响红细胞（RBC）聚集。血浆脂质和脂蛋白也被证明与高胆固醇血症患者的红细胞聚集有关。然而，急性期蛋白是否会促进高脂血症患者的红细胞过度聚集尚不清楚。本研究的主要目的是确定纤维蛋白原（Fib）、触珠蛋白（Hp）、铜蓝蛋白（Cp）、α1-酸性糖蛋白（AGP）、α1-抗胰蛋白酶（AT）、免疫球蛋白G（IgG）和白蛋白（Alb）等急性期蛋白对35例高脂血症患者红细胞聚集的影响。还测定了这些蛋白在32例血脂正常受试者中的影响。
采用激光反射法测量反映红细胞缗钱状形成动力学和红细胞间黏附强度的红细胞聚集参数。进行多变量向前逐步线性回归分析，以研究红细胞聚集与这些急性期蛋白、总胆固醇（TC）、甘油三酯（TG）、高密度（HDL-C）和低密度（LDL-C）脂蛋白胆固醇、年龄、性别、体重指数（BMI）、平均血压（Mpresure）和吸烟习惯之间的关系。在高脂血症患者中，红细胞缗钱状形成动力学与IgG和Hp的线性组合呈正相关（r=0.76，p<0.0001），而在健康受试者中，IgG、吸烟、AGP和性别是显著的独立预测因素（r=0.79，p<0.0001）。预测红细胞间黏附强度的模型在患者中的相关性为0.69（Alb、HDL-C、IgG，p<0.002），在健康个体中的相关性为0.71（AGP、BMI，p<0.0001）。
本研究表明，IgG、Hp、AGP和Alb等急性期蛋白以独立的方式显著影响红细胞聚集水平。红细胞聚集与心血管危险因素之间的密切关联进一步强化了其临床重要性。
