Minamino T, Kitakaze M, Node K, Funaya H, Inoue M, Hori M, Kamada T
First Department of Medicine, Osaka University School of Medicine, Japan.
Am J Physiol. 1996 Dec;271(6 Pt 2):H2622-8. doi: 10.1152/ajpheart.1996.271.6.H2622.
Polymorphonuclear leukocytes (PMNs) can induce endothelium-dependent constriction of vascular rings. Because adenosine inhibits the function of PMNs, we examined the effects of adenosine on the PMN-induced coronary vasoconstriction. We measured changes in the isometric tension of isolated rings of canine coronary arteries suspended in an organ chamber filled with Krebs-Henseleit solution after the addition of autologous PMNs. N-formyl-L-methionyl-leucyl-phenylalanine (FMLP)-stimulated PMNs increased the tension of the coronary artery with the endothelium in a concentration-dependent manner. Treatment of FMLP-stimulated PMNs with adenosine inhibited both the adhesion of PMNs to the endothelium and the PMN-induced vasoconstriction. Stimulation of PMNs with CGS-21680C, but not with cyclohexyladenosine, inhibited both the adhesion of PMNs to the endothelium and the PMN-induced vasoconstriction. However, treatment of coronary arteries with adenosine had no effect on the adherence of PMNs to the endothelium and the PMN-induced constriction. These results suggest that stimulation of adenosine A2a receptors on PMNs may inhibit the PMN-induced vasoconstriction by inhibiting the adhesion of PMNs to the endothelium.
多形核白细胞（PMNs）可诱导血管环的内皮依赖性收缩。由于腺苷可抑制PMNs的功能，我们研究了腺苷对PMN诱导的冠状动脉收缩的影响。在加入自体PMNs后，我们测量了悬浮于充满Krebs-Henseleit溶液的器官浴槽中的犬冠状动脉离体环的等长张力变化。N-甲酰-L-蛋氨酰-亮氨酰-苯丙氨酸（FMLP）刺激的PMNs以浓度依赖性方式增加了有内皮的冠状动脉的张力。用腺苷处理FMLP刺激的PMNs可抑制PMNs与内皮的黏附以及PMN诱导的血管收缩。用CGS-21680C而非环己基腺苷刺激PMNs可抑制PMNs与内皮的黏附以及PMN诱导的血管收缩。然而，用腺苷处理冠状动脉对PMNs与内皮的黏附以及PMN诱导的收缩无影响。这些结果表明，刺激PMNs上的腺苷A2a受体可能通过抑制PMNs与内皮的黏附来抑制PMN诱导的血管收缩。
