Blaise V, Wolf J P, Regnard J, Berthelay S
Faculté de Médecine, Laboratoire de Physiologie-Médicine, Besançon, France.
Cell Mol Biol (Noisy-le-grand). 1996 Dec;42(8):1173-9.
Characterization of human platelet receptors for atrial natriuretic peptide (ANP) has been performed using two different methods. First, intracellular cGMP levels were measured after platelet stimulation with humanANP (hANP) and CNP, in order to detect the presence of ANP-A or ANP-B receptors. The intracellular cGMP content was not modified either after hANP or CNP platelet stimulation and thus indicated the lack of biological active receptors on these cells. Second, displacements of 125I-hANP were similar when induced by hANP or C4-23ANP (specific ligand of clearance receptor). Consequently, these results confirmed that human platelet membranes bore only clearance receptors for atrial natriuretic peptide.
利用两种不同方法对人血小板心房利钠肽（ANP）受体进行了表征。首先，在用人类ANP（hANP）和CNP刺激血小板后，测量细胞内cGMP水平，以检测ANP - A或ANP - B受体的存在。hANP或CNP刺激血小板后，细胞内cGMP含量均未改变，因此表明这些细胞上缺乏生物活性受体。其次，当由hANP或C4 - 23ANP（清除受体的特异性配体）诱导时，125I - hANP的置换情况相似。因此，这些结果证实人血小板膜上仅存在心房利钠肽的清除受体。
