Suda M, Tanaka K, Fukushima M, Natsui K, Yasoda A, Komatsu Y, Ogawa Y, Itoh H, Nakao K
Department of Medicine and Clinical Science, Kyoto University, Graduate School of Medicine 54, Japan.
Biochem Biophys Res Commun. 1996 Jun 5;223(1):1-6. doi: 10.1006/bbrc.1996.0836.
C-type natriuretic peptide (CNP) is a local regulator in the brain and vascular wall. We present data to demonstrate the production and action of CNP in the osteoblast. CNP increased cGMP production, far more potently than atrial natriuretic peptide (ANP) in an osteoblastic cell line, MC3T3-E1. Since ANP and CNP are the ligands for two particulate guanylate cyclases, guanylate cyclase-A (GC-A) and guanylate cyclase-B (GC-B), respectively, these results reveal the expression of GC-B in MC3T3-E1. In addition, CNP mRNA and CNP-like immunoreactivity were detected in cell extracts from MC3T3-E1 and its culture medium, respectively. Both CNP and 8-bromo cGMP dose-dependently decreased [3H]thymidine uptake, without affecting alkaline phosphatase activity. These results indicate that CNP is a novel autocrine/paracrine regulator of osteoblast and suggest the presence of "bone natriuretic peptide system."
C型利钠肽（CNP）是脑和血管壁中的一种局部调节因子。我们提供的数据表明CNP在成骨细胞中的产生及作用。在成骨细胞系MC3T3-E1中，CNP增加环磷酸鸟苷（cGMP）的产生，其效力远高于心房利钠肽（ANP）。由于ANP和CNP分别是两种颗粒型鸟苷酸环化酶——鸟苷酸环化酶-A（GC-A）和鸟苷酸环化酶-B（GC-B）的配体，这些结果揭示了MC3T3-E1中GC-B的表达。此外，分别在MC3T3-E1的细胞提取物及其培养基中检测到了CNP信使核糖核酸（mRNA）和CNP样免疫反应性。CNP和8-溴化cGMP均呈剂量依赖性地降低[3H]胸腺嘧啶核苷摄取，而不影响碱性磷酸酶活性。这些结果表明CNP是成骨细胞一种新的自分泌/旁分泌调节因子，并提示存在“骨利钠肽系统”。
