关于产气克雷伯菌W70菌株的胺氧化酶。

On the amine oxidases of Klebsiella aerogenes strain W70.

作者信息

Cooper R A

机构信息

Department of Biochemistry, University of Leicester, UK.

出版信息

FEMS Microbiol Lett. 1997 Jan 1;146(1):85-9. doi: 10.1111/j.1574-6968.1997.tb10175.x.

DOI:10.1111/j.1574-6968.1997.tb10175.x

PMID:8997710

Abstract

Klebsiella aerogenes W70 was reported previously to produce a membrane-associated tyramine oxidase (TynA) that did not act on 2-phenylethylamine. Subsequently, a gene cloned from K. aerogenes W70 produced a soluble amine oxidase (MaoA) that acted readily on 2-phenylethylamine and tyramine. This enzyme appeared to be equivalent to a 2-phenylethylamine oxidase of Escherichia coli K-12 (MaoA) but was assumed to be the originally described K. aerogenes W70 tyramine oxidase (TynA). However, as described here, whole cells and cell-free extracts of K. aerogenes W70 showed only the tyramine oxidase (TynA) that is inactive against 2-phenylethylamine and not the maoA gene product. It seems that the organism has two amine oxidase genes, tynA and maoA, but only tynA is expressed. Hence, data relating to the expression of the K. aerogenes W70 tynA gene cannot be assumed to apply to the maoA gene of E. coli K-12 because they encode different enzymes.

摘要

先前有报道称产气克雷伯菌W70可产生一种与膜相关的酪胺氧化酶（TynA），该酶对2-苯乙胺无作用。随后，从产气克雷伯菌W70克隆的一个基因产生了一种可溶性胺氧化酶（MaoA），它对2-苯乙胺和酪胺都有很好的作用。这种酶似乎等同于大肠杆菌K-12的2-苯乙胺氧化酶（MaoA），但被认为是最初描述的产气克雷伯菌W70酪胺氧化酶（TynA）。然而，如本文所述，产气克雷伯菌W70的全细胞和无细胞提取物仅显示出对2-苯乙胺无活性的酪胺氧化酶（TynA），而没有maoA基因产物。看来该生物体有两个胺氧化酶基因，tynA和maoA，但只有tynA表达。因此，与产气克雷伯菌W70 tynA基因表达相关的数据不能被认为适用于大肠杆菌K-12的maoA基因，因为它们编码不同的酶。