Hanlon Steven P, Hill Terena K, Flavell Margaret A, Stringfellow Joseph M, Cooper Ronald A
Department of Biochemistry, University of Leicester, Leicester LE1 7RH, UK.
Microbiology (Reading). 1997 Feb;143 ( Pt 2):513-518. doi: 10.1099/00221287-143-2-513.
A gene encoding phenylacetaldehyde dehydrogenase (PAD), the enzyme involved together with a copper-topaquinone-containing amine oxidase in the initial steps of 2-phenylethylamine catabolism, was located at 31.1 min on the Escherichia coli K-12 genetic map. It was immediately adjacent to the gene encoding the amine oxidase but transcribed in the opposite direction. The purified PAD acted almost equally well on phenylacetaldehyde, 4-hydroxyphenylacetaldehyde and 3,4-dihydroxyphenylacetaldehyde. It had a subunit size of 54 kDa and its deduced amino acid sequence was approximately 40% identical to various eukaryotic and prokaryotic aldehyde dehydrogenases. A third gene encoding a positive regulatory protein required for expression of the amine oxidase and PAD genes was located next to the PAD gene. A gene previously located in this position was reported to encode a second amine oxidase but this was not confirmed. The nucleotide sequence from 1447 to 1450 kb on the E. coli K-12 physical map has been determined.
一个编码苯乙醛脱氢酶（PAD）的基因位于大肠杆菌K-12基因图谱的31.1分钟处，该酶与含铜-对苯二醌的胺氧化酶共同参与2-苯乙胺分解代谢的起始步骤。它紧邻编码胺氧化酶的基因，但转录方向相反。纯化后的PAD对苯乙醛、4-羟基苯乙醛和3,4-二羟基苯乙醛的作用效果几乎相同。它的亚基大小为54 kDa，其推导的氨基酸序列与各种真核和原核醛脱氢酶约有40%的同源性。第三个基因编码胺氧化酶和PAD基因表达所需的正调控蛋白，位于PAD基因旁边。先前位于该位置的一个基因据报道编码第二种胺氧化酶，但未得到证实。已确定了大肠杆菌K-12物理图谱上1447至1450 kb的核苷酸序列。
