Monteiro-Neto V, Campos L C, Ferreira A J, Gomes T A, Trabulsi L R
Departamento de Microbiologia, Universidade de São Paulo, Brazil.
FEMS Microbiol Lett. 1997 Jan 1;146(1):123-8. doi: 10.1111/j.1574-6968.1997.tb10181.x.
Seventeen Escherichia coli O111:H12 strains isolated from the feces of children with acute diarrhea were studied with regard to their adherence properties and other virulence characteristics. All strains showed an aggregative adherence pattern to HEp-2 cells and agglutinated bovine and sheep red cells in the presence of mannose. These strains did not have gene sequences homologous to the aggregative adherence fimbria I gene and did not react with any of the DNA probes used to detect other virulence genes in enteropathogens. With one exception, the O111:H12 strains did not induce fluid accumulation in the rabbit ileal loop assay, although 16 of the strains had the enteroaggregative E. coli heat-stable enterotoxin 1 (EAST) gene sequences. A 60-70 MDa plasmid was present in 16 of the strains studied. We conclude that the O111:H12 serotype, one of the first E. coli identified in infantile diarrhea, belongs to the enteroaggregative E. coli category but the genes encoding its adherence phenotype are distinct from those previously described.
对从急性腹泻患儿粪便中分离出的17株大肠杆菌O111:H12菌株的黏附特性及其他毒力特征进行了研究。所有菌株对HEp-2细胞均呈现聚集性黏附模式，且在有甘露糖存在的情况下能凝集牛和羊的红细胞。这些菌株没有与聚集性黏附菌毛I基因同源的基因序列，并且不与用于检测肠道病原菌中其他毒力基因的任何DNA探针发生反应。除1株外，O111:H12菌株在兔回肠袢试验中均未引起液体蓄积，尽管其中16株具有肠聚集性大肠杆菌热稳定肠毒素1（EAST）基因序列。在所研究的菌株中，16株含有一个60 - 70 MDa的质粒。我们得出结论，O111:H12血清型是在婴儿腹泻中最早鉴定出的大肠杆菌之一，属于肠聚集性大肠杆菌类别，但其编码黏附表型的基因与先前描述的不同。
