Koshiishi I, Imanari T
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Anal Chem. 1997 Jan 15;69(2):216-20. doi: 10.1021/ac960704k.
Instabilities of ascorbate and dehydroascorbate throughout sample processing are clearly a significant aspect of quantifying of them. Contents of ascorbate in biological fluids decrease with measurable oxidation occurring within minutes to hours. Similarly, dehydroascorbate disappears with chemical or enzymatic degradation within minutes. The half-life of dehydroascorbate in human heparinized plasma was approximately 2 min. These results indicated that the amount of dehydroascorbate present in sample solutions is a function of both the oxidation of ascorbate and the degradation of dehydroascorbate during the processing of biological fluids. To quantify ascorbate and dehydroascorbate concentrations in biological fluids including circulating blood plasma and urine, we established a high-performance liquid chromatographic method, which requires no pretreatment of sample solutions.
在整个样品处理过程中，抗坏血酸和脱氢抗坏血酸的不稳定性显然是对它们进行定量的一个重要方面。生物流体中抗坏血酸的含量会随着在数分钟到数小时内发生的可测量氧化而降低。同样，脱氢抗坏血酸会在数分钟内因化学或酶促降解而消失。脱氢抗坏血酸在人肝素化血浆中的半衰期约为2分钟。这些结果表明，样品溶液中存在的脱氢抗坏血酸的量是生物流体处理过程中抗坏血酸氧化和脱氢抗坏血酸降解两者的函数。为了定量包括循环血浆和尿液在内的生物流体中的抗坏血酸和脱氢抗坏血酸浓度，我们建立了一种高效液相色谱法，该方法不需要对样品溶液进行预处理。
