使用抗坏血酸氧化酶快速测定血液中抗坏血酸盐和脱氢抗坏血酸盐的直接分光光度法的评估。

Evaluation of a direct spectrophotometric method for the rapid determination of ascorbate and dehydroascorbate in blood using ascorbate oxidase.

作者信息

Wunderling M, Paul H H, Lohmann W

出版信息

Biol Chem Hoppe Seyler. 1986 Oct;367(10):1047-54. doi: 10.1515/bchm3.1986.367.2.1047.

DOI:10.1515/bchm3.1986.367.2.1047

Abstract

The application of a technique for the direct spectrophotometric determination of ascorbate and dehydroascorbate in blood samples is described. The reliability of the test system and various disturbing factors are assessed. It is concluded that the technique possesses a high specificity and that interferences by various biochemicals and metabolic intermediates are low. The lowest ascorbate concentration detectable in plasma by this assay is estimated to be about 1 mumol/l.

摘要

本文描述了一种直接分光光度法测定血样中抗坏血酸盐和脱氢抗坏血酸盐的技术应用。评估了测试系统的可靠性和各种干扰因素。得出的结论是，该技术具有高特异性，各种生化物质和代谢中间体的干扰较低。通过该测定法在血浆中可检测到的最低抗坏血酸盐浓度估计约为1微摩尔/升。