针对两种痘苗病毒抗原产生单特异性抗血清。
Production of monospecific antisera against two vaccinia virus antigens.
作者信息
Gurvin I, Haukenes G
出版信息
Acta Pathol Microbiol Scand C. 1977 Aug;85C(4):297-300. doi: 10.1111/j.1699-0463.1977.tb03645.x.
Abstract
Vaccinia virus antigen was prepared from infected rabbit lung fibroblast monolayers grown in Eagle's medium supplemented with serum obtained from the rabbit going to be used for immunization. Monospecific rabbit antisera against the L and S antigens were produced by immunization with immunoprecipitates cut out from the agarose after quantitative line immunoelectrophoresis using antigen produced in HeLa cells.
摘要
痘苗病毒抗原是从感染的兔肺成纤维细胞单层中制备的，这些细胞在补充了来自即将用于免疫的兔子血清的伊格尔培养基中生长。通过用从使用HeLa细胞产生的抗原进行定量线状免疫电泳后从琼脂糖中切下的免疫沉淀物进行免疫，制备了针对L和S抗原的单特异性兔抗血清。
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