Hutchinson H D, Ziegler D W, Wells D E, Nakano J H
Bull World Health Organ. 1977;55(5):613-23.
Poxvirus antisera adsorbed with "homologous" and "heterologous" poxvirus-infected chorioallantoic membranes (CAM) were differentiated by solid-phase radioimmunoassay (RIA). Mixtures of the antiserum dilutions and infected CAM were added directly (without centrifugation) to poxvirus-infected CAM antigens affixed to wells of microtitration plates. The affixed antigens combined with unadsorbed antibodies, and the cross-reactive antigen-antibody complexes were removed by washing. The results showed that adsorption of an antiserum with variola-, vaccinia-, or uninfected-CAM antigen and subsequent reaction of each in RIA with monkeypox- and uninfected-CAM antigens allowed the identification of antivariola, antivaccinia, or antimonkeypox sera.
用固相放射免疫测定法(RIA)区分用“同源”和“异源”痘病毒感染的绒毛尿囊膜(CAM)吸附的痘病毒抗血清。将抗血清稀释液与感染的CAM的混合物直接(不离心)加入固定在微量滴定板孔中的痘病毒感染的CAM抗原中。固定的抗原与未吸附的抗体结合,通过洗涤去除交叉反应性抗原-抗体复合物。结果表明,用天花、牛痘或未感染的CAM抗原吸附抗血清,随后每种抗血清在RIA中与猴痘和未感染的CAM抗原反应,可以鉴定出抗天花、抗牛痘或抗猴痘血清。