[脂肪酸和某些自旋效应剂对高铁血红蛋白四级结构和自旋状态影响的比较研究]
[Comparative study of the effect of fatty acids and certain spin effectors on quaternary structure and spin state of methemoglobin].
作者信息
Bovdeĭ N A, Kiseleva S N, Kiselev P A
出版信息
Biokhimiia. 1996 Sep;61(9):1570-7.
Cysteine beta-93 SH-groups of horse and bovine methemoglobins (metHb) were modified by N-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)iodoacetamide. Effects of pH, F-, CN-, oleic acid, and sodium dodecyl sulfate on spin and conformational dynamics of the protein were studied by electron and EPR spectroscopy. Oleic acid and CN- had similar effects on both parameters. Relationship between spin and conformational transformations of metHb was elucidated. Effect of oleic acid can be due to increased conformational mobility of C-terminus of metHb beta-chains.
马和牛的高铁血红蛋白（metHb）的半胱氨酸β-93巯基被N-（1-氧基-2,2,6,6-四甲基-4-哌啶基）碘乙酰胺修饰。通过电子光谱和电子顺磁共振光谱研究了pH值、氟离子、氰根离子、油酸和十二烷基硫酸钠对蛋白质自旋和构象动力学的影响。油酸和氰根离子对这两个参数有相似的影响。阐明了高铁血红蛋白自旋与构象转变之间的关系。油酸的作用可能是由于高铁血红蛋白β链C末端构象流动性增加所致。
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