Sznajd J, Naskalski J W, Czarnecka H
Folia Haematol Int Mag Klin Morphol Blutforsch. 1979;106(3):341-50.
Ribonuclease (Ribonucleate nucleotide 2'-transferase E.C. 2.7.7.17) activity in serum of patients with chronic granulocytic leukaemia measured at pH 4.5-6.0 amounts to more than three times of that in serum of healthy subjects. At pH 6.0-8.0 the elevation of ribonuclease activity in serum of patients with chronic granulocytic leukaemia is less pronounced and amounts to about two times of that in normal ones. Using chromatography on CM Sephadex C-50 column, serum ribonuclease of both normal and chronic granulocytic leukaemia patients was separated into five distinct fractions. In serum of healthy subjects ribonuclease fractions denoted I-V contribute to 10; 21; 29; 22, and 18 percent of the total ribonuclease activity. In the serum of patients with chronic granulocytic leukaemia a decrease in ribonuclease fraction III to merely 17 percent and an increase in contribution of fraction IV to 32 percent of total ribonuclease activity could be observed. The comparison of each individual concentration of fraction in normal and leukaemia patients serum reveals, that ribonuclease fraction IV will increase about 3 times. A less pronounced increase could also be found for fractions I, II and V. However, ribonuclease fraction IV may be supposed to carry more than 50 percent of the whole extra load of ribonuclease present in the serum of chronic granulocytic leukaemia patients.
在pH值为4.5 - 6.0时测定慢性粒细胞白血病患者血清中的核糖核酸酶（核糖核酸核苷酸2'-转移酶，E.C. 2.7.7.17）活性，其含量是健康受试者血清中该酶活性的三倍多。在pH值为6.0 - 8.0时，慢性粒细胞白血病患者血清中核糖核酸酶活性的升高不太明显，约为正常受试者血清中该酶活性的两倍。通过在CM Sephadex C - 50柱上进行色谱分析，将正常人和慢性粒细胞白血病患者的血清核糖核酸酶分离为五个不同的组分。在健康受试者的血清中，标记为I - V的核糖核酸酶组分分别占总核糖核酸酶活性的10%、21%、29%、22%和18%。在慢性粒细胞白血病患者的血清中，可以观察到核糖核酸酶组分III降至仅17%，而组分IV对总核糖核酸酶活性的贡献增加到32%。比较正常人和白血病患者血清中各组分的个体浓度发现，核糖核酸酶组分IV增加约3倍。组分I、II和V也有不太明显的增加。然而，可以推测核糖核酸酶组分IV承担了慢性粒细胞白血病患者血清中核糖核酸酶全部额外负荷的50%以上。
