Khatchadourian C, Joly C, Ménézo Y
INSA Unité de Biologie du développement pré-implantatoire, UA INRA 203, Villeurbanne.
Contracept Fertil Sex. 1996 Sep;24(9):650-2.
It is very difficult to have a clear and homogeneous idea of the embryo metabolism. In fact it may vary from one species to another and also according to the embryonic stage: i.e. before and after genomic activation. Basic compounds such as glucose may be toxic, but obviously, it is more the problem of the quantity introduced in the culture media and an unsuitable balance between the metabolites which may impair the embryonic development. At low concentration glucose is actively metabolised by embryos. High levels of amino acids are deleterious (due to release of ammonia), but they are necessary at low concentrations. Addition of serum or other biological fluids is generally useless. Further knowledge on embryo metabolism is necessary to avoid culture medium related delay or developmental blocks. Sequential media are at least partly the answer.
要对胚胎代谢有一个清晰且统一的认识非常困难。事实上，它可能因物种而异，也会根据胚胎发育阶段而有所不同，即基因组激活前后。像葡萄糖这样的基础化合物可能具有毒性，但显然，更主要的问题是培养基中引入的量以及代谢物之间不合适的平衡，这可能会损害胚胎发育。在低浓度下，葡萄糖会被胚胎积极代谢。高浓度的氨基酸是有害的（由于氨的释放），但在低浓度时它们是必需的。添加血清或其他生物液体通常并无用处。为避免与培养基相关的发育延迟或阻滞，有必要进一步了解胚胎代谢。序贯培养基至少在一定程度上解决了这个问题。
