Shimegi S
Faculty of Health and Sport Sciences, Osaka University, Japan.
Calcif Tissue Int. 1996 Feb;58(2):109-13. doi: 10.1007/BF02529732.
Extracellular ATP, and to a lesser extent adenosine, an ATP metabolite, stimulated cell proliferation in osteoblast-like cells (MC3T3-E1). ATP increased cytosolic Ca2+ due to Ca2+ mobilization from intracellular storage in the same concentration range of the nucleotide as that effective for DNA synthesis, suggesting the mediation of the phospholipase C/Ca2+ system in the mitogenic action. Since adenosine induced no Ca2+ mobilization, P2-purinergic receptor appears to be associated with ATP actions. The growth-promoting effect of ATP was not inhibited by H7, a protein kinase C inhibitor, and indomethacin, a cyclooxygenase inhibitor, indicating no involvement of activation of protein kinase C and production of prostaglandins in ATP-induced mitogenic signals. Either ATP or adenosine remarkably and synergistically potentiated platelet derived growth factor-induced DNA synthesis. These findings suggest that extracellular ATP and adenosine may play a physiological role in the regulation of bone formation.
细胞外ATP以及程度稍轻的ATP代谢产物腺苷，可刺激成骨样细胞（MC3T3-E1）的细胞增殖。在与DNA合成有效浓度范围相同的核苷酸浓度下，ATP由于细胞内储存的Ca2+动员而增加了胞质Ca2+，这表明磷脂酶C/Ca2+系统参与了有丝分裂作用的介导。由于腺苷未诱导Ca2+动员，P2-嘌呤能受体似乎与ATP的作用有关。ATP的促生长作用不受蛋白激酶C抑制剂H7和环氧化酶抑制剂吲哚美辛的抑制，这表明蛋白激酶C的激活和前列腺素的产生不参与ATP诱导的有丝分裂信号。ATP或腺苷均可显著且协同增强血小板衍生生长因子诱导的DNA合成。这些发现表明，细胞外ATP和腺苷可能在骨形成的调节中发挥生理作用。
