Semenova N P, Prokudina E N, Chumakov V M
Mol Gen Mikrobiol Virusol. 1996 Jul-Sep(3):11-6.
Previously we demonstrated that in the course of intracellular reproduction of WSN influenza virus strain, part of monomeric nucleoprotein (NP) undergo polymerization into dimers and trimers, which dissociate into monomers after boiling. Further studies showed that different strains of influenza virus are characterized by different degree of NP-oligomerization. Specifically, Duck/ Ukraine/63 (H3N8) and Seal Massacuhsets 1/80 (H7N7) NP monomers are completely transformed into oligomers. As a result of 40-min chase and of prolonged label exposure only NP-oligomers but not monomers can be detected in unboiled samples of infected cells or in virions. NP monomers of A/Duck/Ukraine strain are detectable in unboiled samples only after a short period of labeling. Influenza virus NP oligomers are more hydrophobic than NP monomers. Oligomers are hypothesized to be the native functionally important form of influenza virus NP.
先前我们证明，在WSN流感病毒株的细胞内繁殖过程中，部分单体核蛋白（NP）会聚合成二聚体和三聚体，煮沸后它们会解离成单体。进一步的研究表明，不同的流感病毒株具有不同程度的NP寡聚化特征。具体而言，鸭/乌克兰/63（H3N8）和海豹马萨诸塞州1/80（H7N7）NP单体完全转化为寡聚体。经过40分钟的追踪和长时间的标记暴露后，在未煮沸的感染细胞样本或病毒颗粒中只能检测到NP寡聚体，而检测不到单体。只有在短时间标记后，才能在未煮沸的样本中检测到A/鸭/乌克兰株的NP单体。流感病毒NP寡聚体比NP单体更疏水。据推测，寡聚体是流感病毒NP在功能上重要的天然形式。
