传染性软疣病毒（MCV）与痘苗病毒（VV）基因组组织的相似性：鉴定VV中蛋白激酶2、结构蛋白VP8、RNA聚合酶35 kDa亚基和3β-羟基类固醇脱氢酶基因的MCV同源物。

Similarity in genome organization between Molluscum contagiosum virus (MCV) and vaccinia virus (VV): identification of MCV homologues of the VV genes for protein kinase 2, structural protein VP8, RNA polymerase 35 kDa subunit and 3beta-hydroxysteroid dehydrogenase.

作者信息

Douglass N J, Blake N W, Cream J J, Soteriou B A, Zhang H Y, Theodoridou A, Archard L C

机构信息

Department of Biochemistry, Charing Cross and Westminster Medical School, London, UK.

出版信息

J Gen Virol. 1996 Dec;77 ( Pt 12):3113-20. doi: 10.1099/0022-1317-77-12-3113.

DOI:10.1099/0022-1317-77-12-3113

PMID:9000105

Abstract

Molluscum contagiosum virus (MCV) and vaccinia virus (VV) are serologically unrelated poxviruses with a disparate genome composition (MCV, 66% G+C; VV, 33% G+C). Molecular studies of MCV have been hindered by the inability to propagate the virus in cells cultured in vitro. We sequenced 7765 bp of MCV DNA cloned from four widely spaced regions throughout the MCV genome and identified a total of 11 potential open reading frames (ORF), designated CX1-11. These include MCV homologues of the VV genes encoding protein kinase 2, structural protein VP8, RNA polymerase 35 kDa subunit and 3beta-hydroxysteroid dehydrogenase. The position and orientation of the MCV ORFs was collinear to the VV genome, with the exception of the region around ORF CX11 which is inverted in the MCV genome.

摘要

传染性软疣病毒（MCV）和痘苗病毒（VV）在血清学上无关联，具有不同的基因组组成（MCV，G+C含量为66%；VV，G+C含量为33%）。由于无法在体外培养细胞中繁殖该病毒，对MCV的分子研究受到了阻碍。我们对从MCV基因组中四个间隔较远区域克隆的7765 bp的MCV DNA进行了测序，共鉴定出11个潜在的开放阅读框（ORF），命名为CX1 - 11。这些包括编码蛋白激酶2、结构蛋白VP8、RNA聚合酶35 kDa亚基和3β-羟基类固醇脱氢酶的VV基因的MCV同源物。MCV ORF的位置和方向与VV基因组共线，但ORF CX11周围的区域在MCV基因组中是反向的。