删除痘苗病毒基因中3β-羟基类固醇脱氢酶（A44L）和DNA连接酶（A50R）基因之间的禽痘病毒同源物。

Deletion of fowlpox virus homologues of vaccinia virus genes between the 3 beta-hydroxysteroid dehydrogenase (A44L) and DNA ligase (A50R) genes.

作者信息

Skinner M A, Moore J B, Binns M M, Smith G L, Boursnell M E

机构信息

Institute for Animal Health, Compton Laboratory, Nr. Newbury, Berkshire, U.K.

出版信息

J Gen Virol. 1994 Sep;75 ( Pt 9):2495-8. doi: 10.1099/0022-1317-75-9-2495.

DOI:10.1099/0022-1317-75-9-2495

PMID:8077953

Abstract

A fragment of 4156 bp of fowlpox virus (FPV) genomic DNA contains homologues of vaccinia virus 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD; A44L) and DNA ligase (A50R) genes. The FPV locus has clearly been rearranged relative to that of vaccinia virus as homologues of genes A45R to A49R, including the thymidylate kinase and a gene with homology to superoxide dismutase, are deleted. The deleted genes are replaced by two open reading frames: for a serine proteinase inhibitor with homology to vaccinia virus gene K2L and for a protein with no significant homology to proteins in the databases. In addition, the FPV homologues of A44L and A50R are in the same polarity in FPV whereas they are in opposite polarities in vaccinia virus. Increased 3 beta-HSD activity has been demonstrated in cells infected with either of two different strains of FPV or with canarypox virus.

摘要

禽痘病毒（FPV）基因组DNA的一段4156 bp片段包含痘苗病毒3β-羟基类固醇脱氢酶/δ5-δ4异构酶（3β-HSD；A44L）和DNA连接酶（A50R）基因的同源物。相对于痘苗病毒，FPV基因座明显发生了重排，因为包括胸苷酸激酶和与超氧化物歧化酶具有同源性的一个基因在内的A45R至A49R基因的同源物被删除。被删除的基因被两个开放阅读框取代：一个与痘苗病毒基因K2L具有同源性的丝氨酸蛋白酶抑制剂，以及一个与数据库中的蛋白质无明显同源性的蛋白质。此外，A44L和A50R的FPV同源物在FPV中具有相同的极性，而在痘苗病毒中它们具有相反的极性。在感染两种不同FPV毒株之一或金丝雀痘病毒的细胞中已证明3β-HSD活性增加。