Lin F, Liu P I, McGregor D H
Am J Clin Pathol. 1977 Sep;68(3):372-6. doi: 10.1093/ajcp/68.3.372.
A modified Coombs' mixed cell agglutination reaction was performed on biopsy specimens of 24 patients with squamous cell carcinoma of the larynx. Isoantigens A, B, and H(O) were absent in 15 cases, partially lost in eight, and completely retained in one case. In four cases the initial biopsy specimens were histologically benign yet negative for isoantigens. In subsequent biopsies these showed the presence of carcinoma. The morphologically normal-appearing squamous mucosa adjacent to a carcinoma was found to be devoid of isoantigens in ten cases. The investigation suggested that isoantigenic alteration as detected by mixed cell agglutination reaction was a sensitive sign, since it occurred in squamous mucosa prior to recognizable morphologic change for malignancy. The reaction may represent a functional dedifferentiation in cancerous tissue, analogous to but sometimes preceding morphologic dedifferentiation. The results were comparable to those in previous studies of neoplasms of various other anatomic sites.
对24例喉鳞状细胞癌患者的活检标本进行了改良的库姆斯混合细胞凝集反应。15例中A、B和H（O）同种抗原缺失，8例部分丧失，1例完全保留。4例初始活检标本组织学上为良性，但同种抗原呈阴性。在随后的活检中，这些标本显示有癌存在。在10例病例中，发现癌旁形态学上看似正常的鳞状黏膜缺乏同种抗原。该研究表明，混合细胞凝集反应检测到的同种抗原改变是一个敏感指标，因为它发生在鳞状黏膜出现可识别的恶性形态学改变之前。该反应可能代表癌组织中的一种功能性去分化，类似于形态学去分化，但有时先于形态学去分化出现。结果与先前对其他各种解剖部位肿瘤的研究结果相当。
