Arruda L K, Vailes L D, Platts-Mills T A, Fernandez-Caldas E, Montealegre F, Lin K L, Chua K Y, Rizzo M C, Naspitz C K, Chapman M D
Asthma and Allergic Diseases Center, Department of Internal Medicine, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
Am J Respir Crit Care Med. 1997 Jan;155(1):343-50. doi: 10.1164/ajrccm.155.1.9001334.
In tropical and subtropical regions of the world, allergens produced by Blomia tropicalis are an important cause of IgE-mediated sensitization among patients with asthma. We compared the relative importance of sensitization to the two mite species among asthma patients from Florida, Puerto Rico, and Brazil (n = 83), who were concurrently exposed to B. tropicalis and D. pteronyssinus, with patients from the United States and from the United Kingdom (n = 56) exposed to D. pteronyssinus. In addition, molecular cloning techniques were used to clone and express a major B. tropicalis allergen. There were significant differences between IgE antibody responses to B. tropicalis and D. pteronyssinus that were related to exposure: only 22% of patients exposed to both species had a high ratio (> 10) of IgE D. pteronyssinus:B. tropicalis, whereas 68% of patients exposed only to D. pteronyssinus had a ratio of > 10 (p < 0.001). A major 14-kD allergen (Blo t 5), cloned from a B. tropicalis cDNA library, showed 43% sequence homology to D. pteronyssinus Der p 5. Recombinant Blo t 5 produced in E. coli reacted with 45 to 69% of sera from B. tropicalis-allergic asthmatics and induced positive immediate skin tests at 10(-3) to 1 microg/ml. In vivo and in vitro comparisons of IgE responses to B. tropicalis, D. pteronyssinus, rBlo t 5, and rDer p 5, showed that B. tropicalis has unique allergens that cause specific IgE responses. The results suggest that B. tropicalis is an independent cause of sensitization and that use of recombinant Blo t 5 should lead to a better understanding of the role of B. tropicalis in causing asthma in tropical environments.
在世界热带和亚热带地区，热带果螨产生的过敏原是哮喘患者中IgE介导致敏反应的一个重要原因。我们比较了来自佛罗里达州、波多黎各和巴西（n = 83）同时接触热带果螨和屋尘螨的哮喘患者，与来自美国和英国（n = 56）仅接触屋尘螨的患者对这两种螨类致敏的相对重要性。此外，运用分子克隆技术克隆并表达了一种主要的热带果螨过敏原。对热带果螨和屋尘螨的IgE抗体反应存在与接触相关的显著差异：同时接触这两种螨的患者中，只有22%的人IgE对屋尘螨与热带果螨的比值较高（> 10），而仅接触屋尘螨的患者中有68%的人该比值> 10（p < 0.001）。从热带果螨cDNA文库中克隆出的一种主要的14-kD过敏原（Blo t 5），与屋尘螨的Der p 5有43%的序列同源性。在大肠杆菌中产生的重组Blo t 5与45%至69%的热带果螨过敏哮喘患者血清发生反应，并在10⁻³至1微克/毫升时诱导出阳性即刻皮肤试验。对热带果螨、屋尘螨、重组Blo t 5和重组Der p 5的IgE反应进行的体内和体外比较表明，热带果螨具有引发特异性IgE反应的独特过敏原。结果表明，热带果螨是致敏的一个独立原因，使用重组Blo t 5应能更好地理解热带果螨在热带环境中引发哮喘的作用。
