Khan J, Iiboshi Y, Nezu R, Chen K, Cui L, Yoshida H, Wasa M, Fukuzawa M, Kamata S, Takagi Y, Okada A
Department of Pediatric Surgery, Osaka University Medical School, Japan.
JPEN J Parenter Enteral Nutr. 1997 Jan-Feb;21(1):31-5. doi: 10.1177/014860719702100131.
Dysfunction of the intestinal barrier, as evidenced by increased intestinal permeability and bacterial translocation, has been reported under total parenteral nutrition (TPN). However, the role of Peyer's patches on the intestinal barrier in TPN is not well understood. We investigated whether TPN alters the uptake of microparticles by the follicle-associated epithelium of Peyer's patches.
Twenty rats were divided into two groups, a control group and a TPN group. Fluorescent polystyrene latex beads, 3.2 +/- 0.2 microns in diameter, were used as a probe for measuring the uptake by Peyer's patches. After 1 week of consuming either the control or TPN diet, rats were killed. On the day of killing, 0.1 mL of latex beads solution was injected into a 1-cm length of ileal loop, within 10 cm of the ileocecal valve. Samples were taken after 30 minutes of injection, sectioned by cryostat, and then viewed under a fluorescent microscope. Follicle-associated epithelial length and particles were counted using a confocal laser scanning microscope. The number of particles within each compartment was standardized per unit length of epithelium of Peyer's patches.
Particle numbers within Peyer's patch dome of the TPN group were significantly increased compared with those of the control group (p < .01).
These data suggest that dysfunction of the intestinal barrier in TPN might be associated with a change of uptake by Peyer's patches.
全肠外营养（TPN）状态下存在肠屏障功能障碍，表现为肠通透性增加和细菌易位。然而，TPN状态下派尔集合淋巴结对肠屏障的作用尚不清楚。我们研究了TPN是否会改变派尔集合淋巴结滤泡相关上皮对微粒的摄取。
将20只大鼠分为两组，即对照组和TPN组。使用直径为3.2±0.2微米的荧光聚苯乙烯乳胶珠作为测量派尔集合淋巴结摄取的探针。在给予对照或TPN饮食1周后，处死大鼠。处死当天，将0.1 mL乳胶珠溶液注入距回盲瓣10 cm内1 cm长的回肠肠袢。注射30分钟后取样，用低温恒温器切片，然后在荧光显微镜下观察。使用共聚焦激光扫描显微镜计数滤泡相关上皮长度和微粒。将每个隔室内的微粒数量按派尔集合淋巴结上皮单位长度进行标准化。
TPN组派尔集合淋巴结圆顶内的微粒数量与对照组相比显著增加（p <.01）。
这些数据表明，TPN状态下的肠屏障功能障碍可能与派尔集合淋巴结摄取的改变有关。
