精子凝集单克隆抗体对家兔的避孕作用。

Contraceptive effect of sperm-agglutinating monoclonal antibodies in rabbits.

作者信息

Castle P E, Whaley K J, Hoen T E, Moench T R, Cone R A

机构信息

Thomas C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218, USA.

出版信息

Biol Reprod. 1997 Jan;56(1):153-9. doi: 10.1095/biolreprod56.1.153.

DOI:10.1095/biolreprod56.1.153

PMID:9002644

Abstract

Immune infertility in humans correlates clinically with the presence of anti-sperm antibodies that trap (agglutinate) sperm in semen and cervical mucus. To test whether sperm-agglutinating antibodies can be effective contraceptive agents, several mouse anti-rabbit sperm (MARS) sperm-agglutinating monoclonal antibodies (mAbs) were developed that rapidly and completely agglutinate sperm: MARS-M3 (IgM), MARS-G16 (IgG3), and MARS-G17 (IgG3). Contraceptive efficacy of these mAbs was tested by mixing the mAb with 0.1 ml semen (approximately 1/5 of a whole ejaculate) immediately before artificially inseminating rabbits paracervically. This paracervical dose of semen provided a rigorous test since it delivered several thousand times more fertilizing doses than does a human ejaculate. All of the mAbs were contraceptively effective, and MARS-G16 reduced the number of fetuses per animal by 88% and 95% with doses of 150 microg and 2 mg, respectively. The contraceptive efficacy of the MARS mAbs in the rabbit suggests that human sperm-agglutinating mAbs may be effective agents for vaginal contraception in humans.

摘要

人类免疫性不孕在临床上与抗精子抗体的存在相关，这些抗体会在精液和宫颈黏液中捕获（凝集）精子。为了测试精子凝集抗体是否可作为有效的避孕剂，研究人员研发了几种小鼠抗兔精子（MARS）精子凝集单克隆抗体（mAb），它们能快速且完全地凝集精子：MARS-M3（IgM）、MARS-G16（IgG3）和MARS-G17（IgG3）。在对兔子进行宫颈旁人工授精前，将这些单克隆抗体与0.1毫升精液（约为一次射精总量的1/5）混合，以此测试它们的避孕效果。这种宫颈旁输精剂量的精液提供了一个严格的测试，因为它输送的受精剂量比人类射精量多出数千倍。所有的单克隆抗体都具有避孕效果，MARS-G16分别使用150微克和2毫克剂量时，使每只动物的胎儿数量减少了88%和95%。MARS单克隆抗体在兔子身上的避孕效果表明，人类精子凝集单克隆抗体可能是人类阴道避孕的有效药物。