Mellonig J T
Department of Periodontics, University of Texas Health Science Center at San Antonio 78284-7894, USA.
Pract Periodontics Aesthet Dent. 1995 Aug;7(6):13-22; quiz 23.
This article is a comprehensive review of the safety of currently used freeze-dried bone allografts. The development of exclusionary criteria for donor procurement is discussed along with the steps taken in processing. The processing includes harvesting in a sterile manner, repeated washings, immersion in ethanol, freezing in liquid nitrogen, freeze-drying, demineralization, and vacuum sealing. The statistical data of laboratory testing for the HIV virus is presented, and the advantages and disadvantages of the ELISA and the PCR tests are outlined. There are four known cases of HIV transmission from bone allograft donors, and the circumstances of these four transmissions are discussed. An overview of hepatitis and other infectious diseases in bone allografts completes the review. The learning objective of this article is to update reader knowledge of measures taken to ensure safety in the utilization of DFDB allografts.
本文是对当前使用的冻干骨同种异体移植安全性的全面综述。文中讨论了供体获取的排除标准的制定以及处理过程中采取的步骤。处理过程包括无菌采集、反复冲洗、浸入乙醇、液氮冷冻、冻干、脱矿质和真空密封。列出了针对艾滋病毒的实验室检测统计数据，并概述了酶联免疫吸附测定（ELISA）和聚合酶链反应（PCR）检测的优缺点。有4例已知的骨同种异体移植供体传播艾滋病毒的病例，并讨论了这4次传播的情况。对骨同种异体移植中的肝炎和其他传染病进行了概述，从而完成了本综述。本文的学习目标是更新读者关于确保冻干脱矿骨同种异体移植使用安全所采取措施的知识。
