R&D Institute, Korea Tooth Bank, 622 Eonju-ro, Gangnam-gu, Seoul, 06101, South Korea.
Oral Oncology Clinic Research Institute and Hospital National Cancer Center, 323 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10408, South Korea.
J Transl Med. 2018 Dec 12;16(1):351. doi: 10.1186/s12967-018-1719-9.
Demineralized dentin matrix (DDM) is commonly used as a bone-graft substitute. This study measured and compared human hepatitis B viruses (HBV) DNA in fresh dentin to that of dentin processed into DDM extracted during dental treatment from HBV-infected patients. The hypothesis was that the processing procedure for DDM would inactivate or eliminate HBV in the dentin matrix obtained from infected patients.
Dentin from eighteen HBV-infected patients was collected and each dentin specimen was divided into two fragments. One fragment was used before processing as fresh dentin (control group) and the other was processed into DDM (experimental group). DNA was extracted and purified from each fresh and processed dentin specimen and the HBV DNA copy number quantitated by real time polymerase chain reaction. The HBV DNA copy number in the fresh dentin specimens were compared relative to serologic test results. The second parameter was to evaluate the effectiveness of the processing procedure (defatting, demineralization, freeze-drying, and sterilization) to inactivate or eliminate HBV by comparing the DNA copy number in the processed DDM with that in the matched fresh dentin specimens. All results were analyzed using Mann-Whitney U test to compare numerical measurements between groups and differences were considered statistically significant at P-values less than 0.05.
The presence of HBV DNA was detected in 55.56% (10/18) of the fresh dentin specimens. For the ten HBV DNA-positive fresh dentin specimens, HBV DNA was detected in two (20%) of the matched processed dentin specimens. The copy number of HBV DNA in the two positive processed dentin specimens was 1.79 and 4.03, which were statistically lower than that of the fresh dentin specimens (P = 0.0167).
The results from this study suggested that fresh dentin may be a carrier of HBV and that the procedure used to generate DDM extensively reduced the levels of HBV DNA. Further studies are needed to evaluate the infectivity of HBV in processed dentin.
脱矿牙本质基质（DDM）通常被用作骨移植物替代物。本研究测量并比较了来自乙型肝炎病毒（HBV）感染患者在牙治疗过程中提取的新鲜牙本质和加工成 DDM 的牙本质中的人 HBV DNA。假设是 DDM 的加工过程会使来自感染患者的牙本质基质中的 HBV 失活或消除。
收集了来自 18 名 HBV 感染患者的牙本质，每个牙本质标本被分为两个部分。一个部分在加工前用作新鲜牙本质（对照组），另一个部分加工成 DDM（实验组）。从每个新鲜和加工的牙本质标本中提取并纯化 DNA，并通过实时聚合酶链反应定量 HBV DNA 拷贝数。将新鲜牙本质标本中的 HBV DNA 拷贝数与血清学检测结果进行比较。第二个参数是通过比较加工后的 DDM 中的 DNA 拷贝数与匹配的新鲜牙本质标本中的 DNA 拷贝数，评估加工过程（脱脂、脱矿、冻干和灭菌）对 HBV 失活或消除的有效性。所有结果均采用 Mann-Whitney U 检验进行分析，以比较组间数值测量值，组间差异具有统计学意义（P 值小于 0.05）。
在 55.56%（10/18）的新鲜牙本质标本中检测到 HBV DNA 的存在。在 10 个 HBV DNA 阳性新鲜牙本质标本中，在两个（20%）匹配的加工牙本质标本中检测到 HBV DNA。两个阳性加工牙本质标本中的 HBV DNA 拷贝数分别为 1.79 和 4.03，明显低于新鲜牙本质标本（P=0.0167）。
本研究结果表明，新鲜牙本质可能是 HBV 的载体，用于生成 DDM 的过程广泛降低了 HBV DNA 的水平。需要进一步研究来评估加工牙本质中 HBV 的感染性。
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